Emodin Induces Apoptotic Death in Murine Myelomonocytic Leukemia WEHI-3 Cells In Vitro and Enhances Phagocytosis in Leukemia Mice In Vivo
Joint Authors
Lai, Tung-Yuan
Chang, Yuan-Chang
Ma, Chia-Yu
Chung, Jing-Gung
Chen, Hung-Yi
Chueh, Fu-Shin
Yang, Jai-Sing
Chiang, Jo-Hua
Huang, Wen-Wen
Lu, Chi-Cheng
Yu, Chun-Shu
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-05-10
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Emodin is one of major compounds in rhubarb (Rheum palmatum L.), a plant used as herbal medicine in Chinese population.
Although many reports have shown that emodin exhibits anticancer activity in many tumor cell types, there is no available information addressing emodin-affected apoptotic responses in the murine leukemia cell line (WEHI-3) and modulation of the immune response in leukemia mice.
We investigated that emodin induced cytotoxic effects in vitro and affected WEHI-3 cells in vivo.
This study showed that emodin decreased viability and induced DNA fragmentation in WEHI-3 cells.
Cells after exposure to emodin for 24 h have shown chromatin condensation and DNA damage.
Emodin stimulated the productions of ROS and Ca2+ and reduced the level of ΔΨm by flow cytometry.
Our results from Western blotting suggest that emodin triggered apoptosis of WEHI-3 cells through the endoplasmic reticulum (ER) stress, caspase cascade-dependent and -independent mitochondrial pathways.
In in vivo study, emodin enhanced the levels of B cells and monocytes, and it also reduced the weights of liver and spleen compared with leukemia mice.
Emodin promoted phagocytic activity by monocytes and macrophages in comparison to the leukemia mice group.
In conclusions, emodin induced apoptotic death in murine leukemia WEHI-3 cells and enhanced phagocytosis in the leukemia animal model.
American Psychological Association (APA)
Chang, Yuan-Chang& Lai, Tung-Yuan& Yu, Chun-Shu& Chen, Hung-Yi& Yang, Jai-Sing& Chueh, Fu-Shin…[et al.]. 2011. Emodin Induces Apoptotic Death in Murine Myelomonocytic Leukemia WEHI-3 Cells In Vitro and Enhances Phagocytosis in Leukemia Mice In Vivo. Evidence-Based Complementary and Alternative Medicine،Vol. 2011, no. 2011, pp.1-13.
https://search.emarefa.net/detail/BIM-478443
Modern Language Association (MLA)
Chang, Yuan-Chang…[et al.]. Emodin Induces Apoptotic Death in Murine Myelomonocytic Leukemia WEHI-3 Cells In Vitro and Enhances Phagocytosis in Leukemia Mice In Vivo. Evidence-Based Complementary and Alternative Medicine No. 2011 (2011), pp.1-13.
https://search.emarefa.net/detail/BIM-478443
American Medical Association (AMA)
Chang, Yuan-Chang& Lai, Tung-Yuan& Yu, Chun-Shu& Chen, Hung-Yi& Yang, Jai-Sing& Chueh, Fu-Shin…[et al.]. Emodin Induces Apoptotic Death in Murine Myelomonocytic Leukemia WEHI-3 Cells In Vitro and Enhances Phagocytosis in Leukemia Mice In Vivo. Evidence-Based Complementary and Alternative Medicine. 2011. Vol. 2011, no. 2011, pp.1-13.
https://search.emarefa.net/detail/BIM-478443
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-478443