Keratinase Production by Three Bacillus spp. Using Feather Meal and Whole Feather as Substrate in a Submerged Fermentation
Joint Authors
Mazotto, Ana Maria
Paraguai de Souza, Edilma
Vermelho, Alane Beatriz
de Lima, Marcos Fábio
Couri, Sonia
Coelho, Rosalie Reed Rodrigues
Cedrola, Sabrina Martins Lage
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-08-01
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Three Bacillus species (B.
subtilis LFB-FIOCRUZ 1270, B.
subtilis LFB-FIOCRUZ 1273, and B.
licheniformis LFB-FIOCRUZ 1274), isolated from the poultry industry, were evaluated for keratinase production using feathers or feather meal as the sole carbon and nitrogen sources in a submerged fermentation.
The three Bacillus spp.
produced extracellular keratinases and peptidases after 7 days.
Feather meal was the best substrate for keratinase and peptidase production in B.
subtilis 1273, with 412 U/mL and 463 U/ml.
The three strains were able to degrade feather meal (62–75%) and feather (40–95%) producing 3.9–4.4 mg/ml of soluble protein in feather meal medium and 1.9–3.3 mg/ml when feather medium was used.
The three strains produced serine peptidases with keratinase and gelatinase activity.
B.
subtilis 1273 was the strain which exhibited the highest enzymatic activity.
American Psychological Association (APA)
Mazotto, Ana Maria& Coelho, Rosalie Reed Rodrigues& Cedrola, Sabrina Martins Lage& de Lima, Marcos Fábio& Couri, Sonia& Paraguai de Souza, Edilma…[et al.]. 2011. Keratinase Production by Three Bacillus spp. Using Feather Meal and Whole Feather as Substrate in a Submerged Fermentation. Enzyme Research،Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-478462
Modern Language Association (MLA)
Mazotto, Ana Maria…[et al.]. Keratinase Production by Three Bacillus spp. Using Feather Meal and Whole Feather as Substrate in a Submerged Fermentation. Enzyme Research No. 2011 (2011), pp.1-7.
https://search.emarefa.net/detail/BIM-478462
American Medical Association (AMA)
Mazotto, Ana Maria& Coelho, Rosalie Reed Rodrigues& Cedrola, Sabrina Martins Lage& de Lima, Marcos Fábio& Couri, Sonia& Paraguai de Souza, Edilma…[et al.]. Keratinase Production by Three Bacillus spp. Using Feather Meal and Whole Feather as Substrate in a Submerged Fermentation. Enzyme Research. 2011. Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-478462
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-478462