Feasibility of Repurposing the Polyanionic Microbicide, PPCM, for Prophylaxis against HIV Transmission during ART
Joint Authors
Feathergill, Kenneth A.
Jackson, Erin M.
Rawlins, Richard G.
Brown, David
Anderson, Robert A.
Bremer, James W.
Morack, Ralph
Source
ISRN Obstetrics and Gynecology
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-11-28
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
HIV-serodiscordant couples wishing to conceive often seek assisted reproduction, during which spermatozoa from infected men are washed to minimize the risk of HIV transmission to partner and fetus.
We sought to improve this method by adding a microbicide, PPCM, as an HIV prophylactic.
HIV-1 (BaL) inhibition by PPCM appears irreversible and independent of added Ca2+.
Without added Ca2+, PPCM (≤10 mg/mL, ≤90 min), a stimulus of Ca2+-dependent acrosomal loss, has no effect on sperm motility, forward progression, or acrosomal status.
PPCM-treated (10 mg/mL) sperm retain their ability to acrosome react when Ca2+ is added.
Sperm DNA integrity/function is unaffected by PPCM (≤10 mg/mL).
Adding PPCM (5 mg/mL, 30 min) to washing media reduces infectivity (viral antigen p24 and RNA) of ex-vivo HIV-infected semen by 3-4 Logs compared with washing alone.
Sperm washing with appropriate extracellular Ca2+ levels and PPCM is significantly more effective than washing alone at reducing HIV infectivity.
American Psychological Association (APA)
Anderson, Robert A.& Brown, David& Jackson, Erin M.& Feathergill, Kenneth A.& Bremer, James W.& Morack, Ralph…[et al.]. 2010. Feasibility of Repurposing the Polyanionic Microbicide, PPCM, for Prophylaxis against HIV Transmission during ART. ISRN Obstetrics and Gynecology،Vol. 2011, no. 2011, pp.1-13.
https://search.emarefa.net/detail/BIM-478523
Modern Language Association (MLA)
Anderson, Robert A.…[et al.]. Feasibility of Repurposing the Polyanionic Microbicide, PPCM, for Prophylaxis against HIV Transmission during ART. ISRN Obstetrics and Gynecology No. 2011 (2011), pp.1-13.
https://search.emarefa.net/detail/BIM-478523
American Medical Association (AMA)
Anderson, Robert A.& Brown, David& Jackson, Erin M.& Feathergill, Kenneth A.& Bremer, James W.& Morack, Ralph…[et al.]. Feasibility of Repurposing the Polyanionic Microbicide, PPCM, for Prophylaxis against HIV Transmission during ART. ISRN Obstetrics and Gynecology. 2010. Vol. 2011, no. 2011, pp.1-13.
https://search.emarefa.net/detail/BIM-478523
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-478523