Quantification of Amiridine in Human Plasma by High-Performance Liquid Chromatography Coupled with Electrospray Tandem Mass Spectrometry
Joint Authors
Miroshnichenko, Igor I.
Platova, Angelina I.
Source
Chromatography Research International
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-09-22
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
The aim of this study was to develop and validate a high-performance liquid chromatographic-tandem mass spectrometric (LC-MS/MS) method for analysis of the amiridine in human plasma.
The analyte and internal standard (IS), zolpidem, were extracted from human plasma by solid phase extraction (SPE with SOLA cartridges) and separated on a Zorbax SB-C18 column using methanol and 0.2% formic acid in water as mobile phase.
Detection was performed using an electrospray ionization source and mass spectrometric positive multireaction monitoring mode (+MRM) at a voltage capillary of +2000 V.
The assay was linear over the concentration range 0.5–200 ng/mL with the lowest limit of quantification (LLOQ) of 0.5 ng/mL.
The method also afforded satisfactory results in terms of the sensitivity, specificity, precision (intra- and interday CV<12%), accuracy, recovery, and the stability of the analyte under various conditions.
The method can be successfully applied to pharmacokinetic studies.
American Psychological Association (APA)
Miroshnichenko, Igor I.& Platova, Angelina I.. 2013. Quantification of Amiridine in Human Plasma by High-Performance Liquid Chromatography Coupled with Electrospray Tandem Mass Spectrometry. Chromatography Research International،Vol. 2013, no. 2013, pp.1-5.
https://search.emarefa.net/detail/BIM-478574
Modern Language Association (MLA)
Miroshnichenko, Igor I.& Platova, Angelina I.. Quantification of Amiridine in Human Plasma by High-Performance Liquid Chromatography Coupled with Electrospray Tandem Mass Spectrometry. Chromatography Research International No. 2013 (2013), pp.1-5.
https://search.emarefa.net/detail/BIM-478574
American Medical Association (AMA)
Miroshnichenko, Igor I.& Platova, Angelina I.. Quantification of Amiridine in Human Plasma by High-Performance Liquid Chromatography Coupled with Electrospray Tandem Mass Spectrometry. Chromatography Research International. 2013. Vol. 2013, no. 2013, pp.1-5.
https://search.emarefa.net/detail/BIM-478574
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-478574