Quantification of Amiridine in Human Plasma by High-Performance Liquid Chromatography Coupled with Electrospray Tandem Mass Spectrometry

Joint Authors

Miroshnichenko, Igor I.
Platova, Angelina I.

Source

Chromatography Research International

Issue

Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-5, 5 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2013-09-22

Country of Publication

Egypt

No. of Pages

5

Main Subjects

Chemistry

Abstract EN

The aim of this study was to develop and validate a high-performance liquid chromatographic-tandem mass spectrometric (LC-MS/MS) method for analysis of the amiridine in human plasma.

The analyte and internal standard (IS), zolpidem, were extracted from human plasma by solid phase extraction (SPE with SOLA cartridges) and separated on a Zorbax SB-C18 column using methanol and 0.2% formic acid in water as mobile phase.

Detection was performed using an electrospray ionization source and mass spectrometric positive multireaction monitoring mode (+MRM) at a voltage capillary of +2000 V.

The assay was linear over the concentration range 0.5–200 ng/mL with the lowest limit of quantification (LLOQ) of 0.5 ng/mL.

The method also afforded satisfactory results in terms of the sensitivity, specificity, precision (intra- and interday CV<12%), accuracy, recovery, and the stability of the analyte under various conditions.

The method can be successfully applied to pharmacokinetic studies.

American Psychological Association (APA)

Miroshnichenko, Igor I.& Platova, Angelina I.. 2013. Quantification of Amiridine in Human Plasma by High-Performance Liquid Chromatography Coupled with Electrospray Tandem Mass Spectrometry. Chromatography Research International،Vol. 2013, no. 2013, pp.1-5.
https://search.emarefa.net/detail/BIM-478574

Modern Language Association (MLA)

Miroshnichenko, Igor I.& Platova, Angelina I.. Quantification of Amiridine in Human Plasma by High-Performance Liquid Chromatography Coupled with Electrospray Tandem Mass Spectrometry. Chromatography Research International No. 2013 (2013), pp.1-5.
https://search.emarefa.net/detail/BIM-478574

American Medical Association (AMA)

Miroshnichenko, Igor I.& Platova, Angelina I.. Quantification of Amiridine in Human Plasma by High-Performance Liquid Chromatography Coupled with Electrospray Tandem Mass Spectrometry. Chromatography Research International. 2013. Vol. 2013, no. 2013, pp.1-5.
https://search.emarefa.net/detail/BIM-478574

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-478574