Biofilm Formation among Clinical and Food Isolates of Listeria monocytogenes
Joint Authors
Borges, Sandra
Barbosa, Joana
Santos, Isabel
Almeida, Gonçalo
Camilo, Ruth
Ferreira, Vânia
Teixeira, Paula
Silva, Joana
Magalhães, Rui
Source
International Journal of Microbiology
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-12-29
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Medicine
Abstract EN
Objective.
A total of 725 Listeria monocytogenes isolates, 607 from various foods and 118 from clinical cases of listeriosis, were investigated concerning their ability to form biofilms, at 4°C during 5 days and at 37°C during 24 h.
Methods.
Biofilm production was carried out on polystyrene tissue culture plates.
Five L.
monocytogenes isolates were tested for biofilm formation after being exposed to acidic and osmotic stress conditions.
Results.
Significant differences (P<0.01) between clinical and food isolates were observed.
At 37°C for 24 h, most food isolates were classified as weak or moderate biofilm formers whereas all the clinical isolates were biofilm producers, although the majority were weak.
At 4°C during 5 days, 65 and 59% isolates, from food and clinical cases, respectively, were classified as weak.
After both sublethal stresses, at 37°C just one of the five isolates tested was shown to be more sensitive to subsequent acidic exposure.
However, at 4°C both stresses did not confer either sensitivity or resistance.
Conclusions.
Significant differences between isolates origin, temperature, and sublethal acidic stress were observed concerning the ability to form biofilms.
Strain, origin, and environmental conditions can determine the level of biofilm production by L.
monocytogenes isolates.
American Psychological Association (APA)
Barbosa, Joana& Borges, Sandra& Camilo, Ruth& Magalhães, Rui& Ferreira, Vânia& Santos, Isabel…[et al.]. 2013. Biofilm Formation among Clinical and Food Isolates of Listeria monocytogenes. International Journal of Microbiology،Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-478595
Modern Language Association (MLA)
Barbosa, Joana…[et al.]. Biofilm Formation among Clinical and Food Isolates of Listeria monocytogenes. International Journal of Microbiology No. 2013 (2013), pp.1-6.
https://search.emarefa.net/detail/BIM-478595
American Medical Association (AMA)
Barbosa, Joana& Borges, Sandra& Camilo, Ruth& Magalhães, Rui& Ferreira, Vânia& Santos, Isabel…[et al.]. Biofilm Formation among Clinical and Food Isolates of Listeria monocytogenes. International Journal of Microbiology. 2013. Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-478595
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-478595