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The Vacuolar-Type H+-ATPase in Ovine Rumen Epithelium is Regulated by Metabolic Signals
Joint Authors
Kuzinski, Judith
Warnke-Gurgel, Christina
Schweigel, Monika
Zitnan, Rudolf
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-01-04
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
In this study, the effect of metabolic inhibition (MI) by glucose substitution with 2-deoxyglucose (2-DOG) and/or application of antimycin A on ovine rumen epithelial cells (REC) vacuolar-type H+-ATPase (vH+-ATPase) activity was investigated.
Using fluorescent spectroscopy, basal pHi of REC was measured to be 7.3±0.1 in HCO3−-free, glucose-containing NaCl medium.
MI induced a strong pHi reduction (−0.44±0.04 pH units) with a more pronounced effect of 2-DOG compared to antimycin A (−0.30±0.03 versus −0.21±0.03 pH units).
Treatment with foliomycin, a specific vH+-ATPase inhibitor, decreased REC pHi by 0.21±0.05 pH units.
After MI induction, this effect was nearly abolished (−0.03±0.02 pH units).
In addition, membrane-associated localization of vH+-ATPase B subunit disappeared.
Metabolic control of vH+-ATPase involving regulation of its assembly state by elements of the glycolytic pathway could provide a means to adapt REC ATP consumption according to energy availability.
American Psychological Association (APA)
Kuzinski, Judith& Zitnan, Rudolf& Warnke-Gurgel, Christina& Schweigel, Monika. 2010. The Vacuolar-Type H+-ATPase in Ovine Rumen Epithelium is Regulated by Metabolic Signals. Journal of Biomedicine and Biotechnology،Vol. 2010, no. 2010, pp.1-12.
https://search.emarefa.net/detail/BIM-478600
Modern Language Association (MLA)
Kuzinski, Judith…[et al.]. The Vacuolar-Type H+-ATPase in Ovine Rumen Epithelium is Regulated by Metabolic Signals. Journal of Biomedicine and Biotechnology No. 2010 (2010), pp.1-12.
https://search.emarefa.net/detail/BIM-478600
American Medical Association (AMA)
Kuzinski, Judith& Zitnan, Rudolf& Warnke-Gurgel, Christina& Schweigel, Monika. The Vacuolar-Type H+-ATPase in Ovine Rumen Epithelium is Regulated by Metabolic Signals. Journal of Biomedicine and Biotechnology. 2010. Vol. 2010, no. 2010, pp.1-12.
https://search.emarefa.net/detail/BIM-478600
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-478600