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Development of a Reverse Transcription Loop-Mediated Isothermal Amplification Method for the Rapid Detection of Subtype H7N9 Avian Influenza Virus
Joint Authors
Wang, Yunhe
Zeng, Xianying
Shi, Jianzhong
Chen, Hualan
Wang, Xiurong
Bao, Hongmei
Xu, Xiaolong
Zhao, Yuhui
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-02-06
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
A novel influenza A (H7N9) virus has emerged in China.
To rapidly detect this virus from clinical samples, we developed a reverse transcription loop-mediated isothermal amplification (RT-LAMP) method for the detection of the H7N9 virus.
The minimum detection limit of the RT-LAMP assay was 0.01 PFU H7N9 virus, making this method 100-fold more sensitive to the detection of the H7N9 virus than conventional RT-PCR.
The H7N9 virus RT-LAMP assays can efficiently detect different sources of H7N9 influenza virus RNA (from chickens, pigeons, the environment, and humans).
No cross-reactive amplification with the RNA of other subtype influenza viruses or of other avian respiratory viruses was observed.
The assays can effectively detect H7N9 influenza virus RNA in drinking water, soil, cloacal swab, and tracheal swab samples that were collected from live poultry markets, as well as human H7N9 virus, in less than 30 min.
These results suggest that the H7N9 virus RT-LAMP assays were efficient, practical, and rapid diagnostic methods for the epidemiological surveillance and diagnosis of influenza A (H7N9) virus from different resource samples.
American Psychological Association (APA)
Bao, Hongmei& Zhao, Yuhui& Wang, Yunhe& Xu, Xiaolong& Shi, Jianzhong& Zeng, Xianying…[et al.]. 2014. Development of a Reverse Transcription Loop-Mediated Isothermal Amplification Method for the Rapid Detection of Subtype H7N9 Avian Influenza Virus. BioMed Research International،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-478601
Modern Language Association (MLA)
Bao, Hongmei…[et al.]. Development of a Reverse Transcription Loop-Mediated Isothermal Amplification Method for the Rapid Detection of Subtype H7N9 Avian Influenza Virus. BioMed Research International No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-478601
American Medical Association (AMA)
Bao, Hongmei& Zhao, Yuhui& Wang, Yunhe& Xu, Xiaolong& Shi, Jianzhong& Zeng, Xianying…[et al.]. Development of a Reverse Transcription Loop-Mediated Isothermal Amplification Method for the Rapid Detection of Subtype H7N9 Avian Influenza Virus. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-478601
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-478601