Regulation of P-Glycoprotein in Renal Proximal Tubule Epithelial Cells by LPS and TNF-α
Joint Authors
Masereeuw, Rosalinde
Russel, Frans G. M.
Heemskerk, Suzanne
Peters, Janny G. P.
Sagar, Seil
Louisse, Jochem
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-03-09
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
During endotoxemia, the ATP-dependent drug efflux pump P-glycoprotein (Abcb1/P-gp) is upregulated in kidney proximal tubule epithelial cells.
The signaling pathway through which lipopolysaccharide (LPS) or tumor necrosis factor-α (TNF-α) regulates P-gp expression and activity was investigated further in the present study.
Exposure of rat kidney proximal tubule cells to TNF-α alone or TNF-α and LPS increased P-gp gene and protein expression levels and efflux activity, suggesting de novo P-gp synthesis.
Upon exposure to TNF-α in combination with LPS, P-gp activity in renal proximal tubule cells is increased under influence of nitric oxide (NO) produced by inducible NO synthase.
Upon exposure to TNF-α alone, P-gp upregulation seems to involve TLR4 activation and nuclear factor kappaB (NF-κB) translocation, a pathway that is likely independent of NO.
These findings indicate that at least two pathways regulate P-gp expression in the kidney during endotoxemia.
American Psychological Association (APA)
Heemskerk, Suzanne& Peters, Janny G. P.& Louisse, Jochem& Sagar, Seil& Russel, Frans G. M.& Masereeuw, Rosalinde. 2010. Regulation of P-Glycoprotein in Renal Proximal Tubule Epithelial Cells by LPS and TNF-α. Journal of Biomedicine and Biotechnology،Vol. 2010, no. 2010, pp.1-10.
https://search.emarefa.net/detail/BIM-478613
Modern Language Association (MLA)
Heemskerk, Suzanne…[et al.]. Regulation of P-Glycoprotein in Renal Proximal Tubule Epithelial Cells by LPS and TNF-α. Journal of Biomedicine and Biotechnology No. 2010 (2010), pp.1-10.
https://search.emarefa.net/detail/BIM-478613
American Medical Association (AMA)
Heemskerk, Suzanne& Peters, Janny G. P.& Louisse, Jochem& Sagar, Seil& Russel, Frans G. M.& Masereeuw, Rosalinde. Regulation of P-Glycoprotein in Renal Proximal Tubule Epithelial Cells by LPS and TNF-α. Journal of Biomedicine and Biotechnology. 2010. Vol. 2010, no. 2010, pp.1-10.
https://search.emarefa.net/detail/BIM-478613
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-478613