Developmental Stage-Specific Imprinting of IPL in Domestic Pigs (Sus scrofa)
Joint Authors
Li, Li
Hou, Shengping
Liang, Jie
Tian, X. Cindy
Wu, Tongshan
Chen, Yuming
Zhang, Shouquan
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-06-02
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Imprinted in placenta and liver (IPL) gene has been identified as an imprinted gene in the mouse and human.
Its sequence and imprinting status, however, have not been determined in the domestic pigs.
In the present study, a 259 base pair-specific sequence for IPL gene of the domestic pig was obtained and a novel SNP, a T/C transition, was identified in IPL exon 1.
The C allele of this polymorphism was found to be the predominant allele in Landrace,Yorkshire, and Duroc.
The frequency of CC genotype and C allele are different in Duroc as compared with Yorkshire (P=.038 and P=.005, resp.).
Variable imprinting status of this gene was observed in different developmental stages.
For example, it is imprinted in 1-dayold newborns (expressed from the maternal allele), but imprinting was lost in 180-day-old adult (expressed from both parental alleles).
Real-time PCR analysis showed the porcine IPL gene is expressed in all tested eight organ/tissues.
The expression level was significantly higher in spleen, duodenum, lung, and bladder of 180-day-old Lantang adult compared to that in 1-day-old newborns Lantang pigs (P<.05).
In conclusion, the imprinting of the porcine IPL gene is developmental stage and tissue specific.
American Psychological Association (APA)
Hou, Shengping& Chen, Yuming& Liang, Jie& Li, Li& Wu, Tongshan& Tian, X. Cindy…[et al.]. 2010. Developmental Stage-Specific Imprinting of IPL in Domestic Pigs (Sus scrofa). Journal of Biomedicine and Biotechnology،Vol. 2010, no. 2010, pp.1-6.
https://search.emarefa.net/detail/BIM-478810
Modern Language Association (MLA)
Hou, Shengping…[et al.]. Developmental Stage-Specific Imprinting of IPL in Domestic Pigs (Sus scrofa). Journal of Biomedicine and Biotechnology No. 2010 (2010), pp.1-6.
https://search.emarefa.net/detail/BIM-478810
American Medical Association (AMA)
Hou, Shengping& Chen, Yuming& Liang, Jie& Li, Li& Wu, Tongshan& Tian, X. Cindy…[et al.]. Developmental Stage-Specific Imprinting of IPL in Domestic Pigs (Sus scrofa). Journal of Biomedicine and Biotechnology. 2010. Vol. 2010, no. 2010, pp.1-6.
https://search.emarefa.net/detail/BIM-478810
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-478810