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Engineering the Production of Major Catechins by Escherichia coli Carrying Metabolite Genes of Camellia sinensis
Joint Authors
Abdulkarim, S. M.
Umar, Kabir Mustapha
Radu, Son
Abdul Hamid, Azizah
Saari, Nazamid
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-05-01
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Medicine
Information Technology and Computer Science
Abstract EN
A mimicked biosynthetic pathway of catechin metabolite genes from C.
sinensis, consisting of flavanone 3 hydroxylase (F3H), dihydroflavonol reductase (DFR), and leucoanthocyanidin reductase (LCR), was designed and arranged in two sets of constructs: (a) single promoter in front of F3H and ribosome-binding sequences both in front of DFR and LCR; (b) three different promoters with each in the front of the three genes and ribosome-binding sequences at appropriate positions.
Recombinant E.
coli BL (DE3) harbouring the constructs were cultivated for 65 h at 26°C in M9 medium consisting of 40 g/L glucose, 1 mM IPTG, and 3 mM eriodictyol.
Compounds produced were extracted in ethyl acetate in alkaline conditions after 1 h at room temperature and identified by HPLC.
Two of the four major catechins, namely, (−)-epicatechin (0.01 ) and (−)-epicatechin gallate (0.36 mg/L), and two other types ((+)-catechin hydrate (0.13 mg/L) and (−)-catechin gallate (0.04 mg/L)) were successfully produced.
American Psychological Association (APA)
Umar, Kabir Mustapha& Abdulkarim, S. M.& Radu, Son& Abdul Hamid, Azizah& Saari, Nazamid. 2012. Engineering the Production of Major Catechins by Escherichia coli Carrying Metabolite Genes of Camellia sinensis. The Scientific World Journal،Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-478954
Modern Language Association (MLA)
Umar, Kabir Mustapha…[et al.]. Engineering the Production of Major Catechins by Escherichia coli Carrying Metabolite Genes of Camellia sinensis. The Scientific World Journal No. 2012 (2012), pp.1-7.
https://search.emarefa.net/detail/BIM-478954
American Medical Association (AMA)
Umar, Kabir Mustapha& Abdulkarim, S. M.& Radu, Son& Abdul Hamid, Azizah& Saari, Nazamid. Engineering the Production of Major Catechins by Escherichia coli Carrying Metabolite Genes of Camellia sinensis. The Scientific World Journal. 2012. Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-478954
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-478954