![](/images/graphics-bg.png)
Palmitic Acid Induces Production of Proinflammatory Cytokines Interleukin-6, Interleukin-1β, and Tumor Necrosis Factor-α via a NF-κB-Dependent Mechanism in HaCaT Keratinocytes
Joint Authors
Zhang, Qian
Zhang, Jia-an
Xu, Yang
Yin, Zhi-qiang
Zhou, Bing-rong
Wu, Di
Permatasari, Felicia
Luo, Dan
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-08-29
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
To investigate whether palmitic acid can be responsible for the induction of inflammatory processes, HaCaT keratinocytes were treated with palmitic acid at pathophysiologically relevant concentrations.
Secretion levels of interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), NF-κB nuclear translocation, NF-κB activation, Stat3 phosphorylation, and peroxisome proliferator-activated receptor alpha (PPARα) mRNA and protein levels, as well as the cell proliferation ability were measured at the end of the treatment and after 24 hours of recovery.
Pyrrolidine dithiocarbamate (PDTC, a selective chemical inhibitor of NF-κB) and goat anti-human IL-6 polyclonal neutralizing antibody were used to inhibit NF-κB activation and IL-6 production, respectively.
Our results showed that palmitic acid induced an upregulation of IL-6, TNF-α, IL-1β secretions, accompanied by NF-κB nuclear translocation and activation.
Moreover, the effect of palmitic acid was accompanied by PPARα activation and Stat3 phosphorylation.
Palmitic acid-induced IL-6, TNF-α, IL-1β productions were attenuated by NF-κB inhibitor PDTC.
Palmitic acid was administered in amounts able to elicit significant hyperproliferation and can be attenuated by IL-6 blockage.
These data demonstrate for the first time that palmitic acid can stimulate IL-6, TNF-α, IL-1β productions in HaCaT keratinocytes and cell proliferation, thereby potentially contributing to acne inflammation and pilosebaceous duct hyperkeratinization.Erratum to “Palmitic Acid Induces Production of Proinflammatory Cytokines Interleukin-6, Interleukin-1β, and Tumor Necrosis Factor-α via a NF-κB-Dependent Mechanism in HaCaT Keratinocytes”dx.doi.org/10.1155/2014/513027
American Psychological Association (APA)
Zhou, Bing-rong& Zhang, Jia-an& Zhang, Qian& Permatasari, Felicia& Xu, Yang& Wu, Di…[et al.]. 2013. Palmitic Acid Induces Production of Proinflammatory Cytokines Interleukin-6, Interleukin-1β, and Tumor Necrosis Factor-α via a NF-κB-Dependent Mechanism in HaCaT Keratinocytes. Mediators of Inflammation،Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-479082
Modern Language Association (MLA)
Zhou, Bing-rong…[et al.]. Palmitic Acid Induces Production of Proinflammatory Cytokines Interleukin-6, Interleukin-1β, and Tumor Necrosis Factor-α via a NF-κB-Dependent Mechanism in HaCaT Keratinocytes. Mediators of Inflammation No. 2013 (2013), pp.1-11.
https://search.emarefa.net/detail/BIM-479082
American Medical Association (AMA)
Zhou, Bing-rong& Zhang, Jia-an& Zhang, Qian& Permatasari, Felicia& Xu, Yang& Wu, Di…[et al.]. Palmitic Acid Induces Production of Proinflammatory Cytokines Interleukin-6, Interleukin-1β, and Tumor Necrosis Factor-α via a NF-κB-Dependent Mechanism in HaCaT Keratinocytes. Mediators of Inflammation. 2013. Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-479082
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-479082