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Development of a Colloidal Gold Kit for the Diagnosis of Severe Fever with Thrombocytopenia Syndrome Virus Infection
Joint Authors
Xu, Weiwen
Liang, Minyao
Liao, Zhihua
Hao, Fen
Wu, Wei
Gao, Xunian
Luo, Shuhong
Wang, Shiwen
Li, Dexin
Wang, Xianguo
Zhang, Quanfu
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-04-14
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
It is critical to develop a cost-effective detection kit for rapid diagnosis and on-site detection of severe fever with thrombocytopenia syndrome virus (SFTSV) infection.
Here, an immunochromatographic assay (ICA) to detect SFTSV infection is described.
The ICA uses gold nanoparticles coated with recombinant SFTSV for the simultaneous detection of IgG and IgM antibodies to SFTSV.
The ICA was developed and evaluated by using positive sera samples of SFTSV infection (n=245) collected from the CDC of China.
The reference laboratory diagnosis of SFTSV infection was based on the “gold standard”.
The results demonstrated that the positive coincidence rate and negative coincidence rate were determined to be 98.4% and 100% for IgM and 96.7% and 98.6% for IgG, respectively.
The kit showed good selectivity for detection of SFTSV-specific IgG and IgM with no interference from positive sera samples of Japanese encephalitis virus infection, Dengue virus infection, Hantavirus infection, HIV infection, HBV surface antigen, HCV antibody, Mycobacterium tuberculosis antibody, or RF.
Based on these results, the ICS test developed may be a suitable tool for rapid on-site testing for SFTSV infections.
American Psychological Association (APA)
Wang, Xianguo& Zhang, Quanfu& Hao, Fen& Gao, Xunian& Wu, Wei& Liang, Minyao…[et al.]. 2014. Development of a Colloidal Gold Kit for the Diagnosis of Severe Fever with Thrombocytopenia Syndrome Virus Infection. BioMed Research International،Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-479100
Modern Language Association (MLA)
Wang, Xianguo…[et al.]. Development of a Colloidal Gold Kit for the Diagnosis of Severe Fever with Thrombocytopenia Syndrome Virus Infection. BioMed Research International No. 2014 (2014), pp.1-6.
https://search.emarefa.net/detail/BIM-479100
American Medical Association (AMA)
Wang, Xianguo& Zhang, Quanfu& Hao, Fen& Gao, Xunian& Wu, Wei& Liang, Minyao…[et al.]. Development of a Colloidal Gold Kit for the Diagnosis of Severe Fever with Thrombocytopenia Syndrome Virus Infection. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-479100
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-479100