Evaluation of Fructooligosaccharides and Inulins as Potentially Health Benefiting Food Ingredients by HPAEC-PED and MALDI-TOF MS
Joint Authors
Mangia, Alessandro
Borromei, Chiara
Elviri, Lisa
Merusi, Cristiana
Corradini, Claudio
Cavazza, Antonella
Careri, Maria
Source
International Journal of Analytical Chemistry
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2009-03-18
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
This paper describes the complementarity of high-performance anion exchange chromatography coupled with pulsed electrochemical detection (HPAEC-PED) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-TOF-MS) to evaluate commercial available fructans (fructooligosaccharides (FOS) and inulins), having different degrees of polymerization (DP) which are usually employed by food industry as functional ingredients either for their prebiotic properties or as a fat replacer, giving a fat-like mouth feel and texture.
The developed HPAEC-PED methods are able to analyze FOS (fructans with DP 3–10) and inulins (DP ranging from 3 to 80) with a good resolution and relatively short retention times to evaluate structural differences between fructooligosaccharide and inulins and the possible presence of inulooligosaccharides as well as of branching.
To characterize FOS and inulin at different degrees of polymerization and to assure correct molecular assignment, MALDI-TOF MS analysis was also investigated.
The 2,5-dihydroxy benzoic acid (2,5-DHB) was found to be the best matrix for FOS analysis as Actilight and Raftilose P95 products, while 3-aminoquinoline (3-AQ) seems to be the best matrix for inulin with higher DP.
The applicability of the optimized methods to the identification and determination of FOS contained in a symbiotic milk as well as a type of inulin added as functional ingredient to a cooked ham is demonstrated.
American Psychological Association (APA)
Borromei, Chiara& Careri, Maria& Cavazza, Antonella& Corradini, Claudio& Elviri, Lisa& Mangia, Alessandro…[et al.]. 2009. Evaluation of Fructooligosaccharides and Inulins as Potentially Health Benefiting Food Ingredients by HPAEC-PED and MALDI-TOF MS. International Journal of Analytical Chemistry،Vol. 2009, no. 2009, pp.1-9.
https://search.emarefa.net/detail/BIM-479101
Modern Language Association (MLA)
Borromei, Chiara…[et al.]. Evaluation of Fructooligosaccharides and Inulins as Potentially Health Benefiting Food Ingredients by HPAEC-PED and MALDI-TOF MS. International Journal of Analytical Chemistry No. 2009 (2009), pp.1-9.
https://search.emarefa.net/detail/BIM-479101
American Medical Association (AMA)
Borromei, Chiara& Careri, Maria& Cavazza, Antonella& Corradini, Claudio& Elviri, Lisa& Mangia, Alessandro…[et al.]. Evaluation of Fructooligosaccharides and Inulins as Potentially Health Benefiting Food Ingredients by HPAEC-PED and MALDI-TOF MS. International Journal of Analytical Chemistry. 2009. Vol. 2009, no. 2009, pp.1-9.
https://search.emarefa.net/detail/BIM-479101
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-479101