Probing Retroviral and Retrotransposon Genome Structures : The “SHAPE” of Things to Come
Joint Authors
Sztuba-Solinska, Joanna
Le Grice, Stuart F. J.
Source
Molecular Biology International
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-05-17
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
Understanding the nuances of RNA structure as they pertain to biological function remains a formidable challenge for retrovirus research and development of RNA-based therapeutics, an area of particular importance with respect to combating HIV infection.
Although a variety of chemical and enzymatic RNA probing techniques have been successfully employed for more than 30 years, they primarily interrogate small (100–500 nt) RNAs that have been removed from their biological context, potentially eliminating long-range tertiary interactions (such as kissing loops and pseudoknots) that may play a critical regulatory role.
Selective 2′ hydroxyl acylation analyzed by primer extension (SHAPE), pioneered recently by Merino and colleagues, represents a facile, user-friendly technology capable of interrogating RNA structure with a single reagent and, combined with automated capillary electrophoresis, can analyze an entire 10,000-nucleotide RNA genome in a matter of weeks.
Despite these obvious advantages, SHAPE essentially provides a nucleotide “connectivity map,” conversion of which into a 3-D structure requires a variety of complementary approaches.
This paper summarizes contributions from SHAPE towards our understanding of the structure of retroviral genomes, modifications to which technology that have been developed to address some of its limitations, and future challenges.
American Psychological Association (APA)
Sztuba-Solinska, Joanna& Le Grice, Stuart F. J.. 2012. Probing Retroviral and Retrotransposon Genome Structures : The “SHAPE” of Things to Come. Molecular Biology International،Vol. 2012, no. 2012, pp.1-12.
https://search.emarefa.net/detail/BIM-479117
Modern Language Association (MLA)
Sztuba-Solinska, Joanna& Le Grice, Stuart F. J.. Probing Retroviral and Retrotransposon Genome Structures : The “SHAPE” of Things to Come. Molecular Biology International No. 2012 (2012), pp.1-12.
https://search.emarefa.net/detail/BIM-479117
American Medical Association (AMA)
Sztuba-Solinska, Joanna& Le Grice, Stuart F. J.. Probing Retroviral and Retrotransposon Genome Structures : The “SHAPE” of Things to Come. Molecular Biology International. 2012. Vol. 2012, no. 2012, pp.1-12.
https://search.emarefa.net/detail/BIM-479117
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-479117