High Specificity of Quantitative Methylation-Specific PCR Analysis for MGMT Promoter Hypermethylation Detection in Gliomas
Joint Authors
Maiello, Evaristo
Balsamo, Teresa
Bisceglia, Michele
Notarangelo, Angelo
D'Angelo, Vincenzo A.
Catapano, Domenico
la Torre, Antonella
Carella, Massimo
Fazio, Vito Michele
Parisi, Salvatore
Copetti, Massimiliano
Gallo, Antonietta Pia
Valori, Vanna M.
Barbano, Raffaela
Parrella, Paola
Dallapiccola, Bruno
Poeta, Maria Luana
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2009-06-01
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Normal brain tissue from 28 individuals and 50 glioma samples were analyzed by real-time Quantitative Methylation-Specific PCR (QMSP).
Data from this analysis were compared with results obtained on the same samples by MSP.
QMSP analysis demonstrated a statistically significant difference in both methylation level (P=.000009 Mann Whitney Test) and frequencies (P=.0000007, Z-test) in tumour samples as compared with normal brain tissues.
Although QMSP and MSP showed similar sensitivity, the specificity of QMSP analysis was significantly higher (93%; CI95%: 84%–100%) as compared with MSP (64%; 95%CI: 46%–82%).
Our results suggest that QMSP analysis may represent a powerful tool to identify glioma patients that will benefit from alkylating agents chemotherapy.
American Psychological Association (APA)
Parrella, Paola& la Torre, Antonella& Copetti, Massimiliano& Valori, Vanna M.& Barbano, Raffaela& Notarangelo, Angelo…[et al.]. 2009. High Specificity of Quantitative Methylation-Specific PCR Analysis for MGMT Promoter Hypermethylation Detection in Gliomas. Journal of Biomedicine and Biotechnology،Vol. 2009, no. 2009, pp.1-8.
https://search.emarefa.net/detail/BIM-479191
Modern Language Association (MLA)
Parrella, Paola…[et al.]. High Specificity of Quantitative Methylation-Specific PCR Analysis for MGMT Promoter Hypermethylation Detection in Gliomas. Journal of Biomedicine and Biotechnology No. 2009 (2009), pp.1-8.
https://search.emarefa.net/detail/BIM-479191
American Medical Association (AMA)
Parrella, Paola& la Torre, Antonella& Copetti, Massimiliano& Valori, Vanna M.& Barbano, Raffaela& Notarangelo, Angelo…[et al.]. High Specificity of Quantitative Methylation-Specific PCR Analysis for MGMT Promoter Hypermethylation Detection in Gliomas. Journal of Biomedicine and Biotechnology. 2009. Vol. 2009, no. 2009, pp.1-8.
https://search.emarefa.net/detail/BIM-479191
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-479191
