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Cell Matrix Remodeling Ability Shown by Image Spatial Correlation
Joint Authors
Gratton, Enrico
Chiu, Chi-Li
Digman, Michelle A.
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-07-14
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Extracellular matrix (ECM) remodeling is a critical step of many biological and pathological processes.
However, most of the studies to date lack a quantitative method to measure ECM remodeling at a scale comparable to cell size.
Here, we applied image spatial correlation to collagen second harmonic generation (SHG) images to quantitatively evaluate the degree of collagen remodeling by cells.
We propose a simple statistical method based on spatial correlation functions to determine the size of high collagen density area around cells.
We applied our method to measure collagen remodeling by two breast cancer cell lines (MDA-MB-231 and MCF-7), which display different degrees of invasiveness, and a fibroblast cell line (NIH/3T3).
We found distinct collagen compaction levels of these three cell lines by applying the spatial correlation method, indicating different collagen remodeling ability.
Furthermore, we quantitatively measured the effect of Latrunculin B and Marimastat on MDA-MB-231 cell line collagen remodeling ability and showed that significant collagen compaction level decreases with these treatments.
American Psychological Association (APA)
Chiu, Chi-Li& Digman, Michelle A.& Gratton, Enrico. 2013. Cell Matrix Remodeling Ability Shown by Image Spatial Correlation. Journal of Biophysics،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-479220
Modern Language Association (MLA)
Chiu, Chi-Li…[et al.]. Cell Matrix Remodeling Ability Shown by Image Spatial Correlation. Journal of Biophysics No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-479220
American Medical Association (AMA)
Chiu, Chi-Li& Digman, Michelle A.& Gratton, Enrico. Cell Matrix Remodeling Ability Shown by Image Spatial Correlation. Journal of Biophysics. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-479220
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-479220