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Detection and Antibiotic Treatment of Mycoplasma arginini Contamination in a Mouse Epithelial Cell Line Restore Normal Cell Physiology
Joint Authors
Boslett, Brianna
Resnick, Andrew
Nag, Subhra
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-03-20
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Mycoplasma contamination of cultured cell lines is difficult to detect by routine observation.
Infected cells can display normal morphology and the slow growth rate of mycoplasma can delay detection for extended periods of time, compromising experimental results.
Positive identification of mycoplasma typically requires cells to be either fixed and stained for DNA or processed with PCR.
We present a method to detect mycoplasma using live-cell optical microscopy typically used for routine observation of cell cultures.
Images of untreated mycoplasma-infected epithelial cells alongside images of infected cells treated with Plasmocin, a commercially available antibiotic targeted to mycoplasma, are shown.
We found that optical imaging is an effective screening tool for detection of mycoplasma contamination.
Importantly, we found that cells regained normal function after the contamination was cleared.
In conclusion, we present a technique to diagnose probable mycoplasma infections in live cultures without fixation, resulting in faster response times and decreased loss of cell material.
American Psychological Association (APA)
Boslett, Brianna& Nag, Subhra& Resnick, Andrew. 2014. Detection and Antibiotic Treatment of Mycoplasma arginini Contamination in a Mouse Epithelial Cell Line Restore Normal Cell Physiology. BioMed Research International،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-479228
Modern Language Association (MLA)
Boslett, Brianna…[et al.]. Detection and Antibiotic Treatment of Mycoplasma arginini Contamination in a Mouse Epithelial Cell Line Restore Normal Cell Physiology. BioMed Research International No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-479228
American Medical Association (AMA)
Boslett, Brianna& Nag, Subhra& Resnick, Andrew. Detection and Antibiotic Treatment of Mycoplasma arginini Contamination in a Mouse Epithelial Cell Line Restore Normal Cell Physiology. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-479228
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-479228