Human Adipose Tissue Derived Stem Cells Promote Liver Regeneration in a Rat Model of Toxic Injury

Joint Authors

Germann, Guenter
Kolbenschlag, Jonas
Niesen, Willem
Gramley, Felix
Leimer, Uwe
Koellensperger, Eva

Source

Stem Cells International

Issue

Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-10, 10 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2013-11-07

Country of Publication

Egypt

No. of Pages

10

Main Subjects

Natural & Life Sciences (Multidisciplinary)

Abstract EN

In the light of the persisting lack of donor organs and the risks of allotransplantations, the possibility of liver regeneration with autologous stem cells from adipose tissue (ADSC) is an intriguing alternative.

Using a model of a toxic liver damage in Sprague Dawley rats, generated by repetitive intraperitoneal application of retrorsine and allyl alcohol, the ability of human ADSC to support the restoration of liver function was investigated.

A two-thirds hepatectomy was performed, and human ADSC were injected into one remaining liver lobe in group 1 (n = 20).

Injection of cell culture medium performed in group 2 (n = 20) served as control.

Cyclosporine was applied to achieve immunotolerance.

Blood samples were drawn weekly after surgery to determine liver-correlated blood values.

Six and twelve weeks after surgery, animals were sacrificed and histological sections were analyzed.

ADSC significantly raised postoperative albumin (P < 0.017), total protein (P < 0.031), glutamic oxaloacetic transaminase (P < 0.001), and lactate dehydrogenase (P < 0.04) levels compared to injection of cell culture medium alone.

Transplanted cells could be found up to twelve weeks after surgery in histological sections.

This study points towards ADSC being a promising alternative to hepatocyte or liver organ transplantation in patients with severe liver failure.

American Psychological Association (APA)

Koellensperger, Eva& Niesen, Willem& Kolbenschlag, Jonas& Gramley, Felix& Germann, Guenter& Leimer, Uwe. 2013. Human Adipose Tissue Derived Stem Cells Promote Liver Regeneration in a Rat Model of Toxic Injury. Stem Cells International،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-479346

Modern Language Association (MLA)

Koellensperger, Eva…[et al.]. Human Adipose Tissue Derived Stem Cells Promote Liver Regeneration in a Rat Model of Toxic Injury. Stem Cells International No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-479346

American Medical Association (AMA)

Koellensperger, Eva& Niesen, Willem& Kolbenschlag, Jonas& Gramley, Felix& Germann, Guenter& Leimer, Uwe. Human Adipose Tissue Derived Stem Cells Promote Liver Regeneration in a Rat Model of Toxic Injury. Stem Cells International. 2013. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-479346

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-479346