Nonintegrating Lentiviral Vector-Based Vaccine Efficiently Induces Functional and Persistent CD8+ T Cell Responses in Mice
Joint Authors
Vendetti, Silvia
Klotman, Mary E.
Baroncelli, Silvia
Leone, Pasqualina
Michelini, Zuleika
Bona, Roberta
Cara, Andrea
Negri, Donatella R. M.
Spada, Massimo
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-05-19
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
CD8+ T cells are an essential component of an effective host immune response to tumors and viral infections.
Genetic immunization is particularly suitable for inducing CTL responses, because the encoded proteins enter the MHC class I processing pathway through either transgene expression or cross-presentation.
In order to compare the efficiency and persistence of immune response induced by genetic vaccines, BALB/c mice were immunized either twice intramuscularly with DNA plasmid expressing a codon-optimized HIV-1 gp120 Envelope sequence together with murine GM-CSF sequence or with a single immunization using an integrase defective lentiviral vector (IDLV) expressing the same proteins.
Results strongly indicated that the schedule based on IDLV vaccine was more efficient in inducing specific immune response, as evaluated three months after the last immunization by IFNγ ELISPOT in both splenocytes and bone marrow- (BM-) derived cells, chromium release assay in splenocytes, and antibody detection in sera.
In addition, IDLV immunization induced high frequency of polyfunctional CD8+ T cells able to simultaneously produce IFNγ, TNFα, and IL2.
American Psychological Association (APA)
Negri, Donatella R. M.& Michelini, Zuleika& Baroncelli, Silvia& Spada, Massimo& Vendetti, Silvia& Bona, Roberta…[et al.]. 2010. Nonintegrating Lentiviral Vector-Based Vaccine Efficiently Induces Functional and Persistent CD8+ T Cell Responses in Mice. Journal of Biomedicine and Biotechnology،Vol. 2010, no. 2010, pp.1-7.
https://search.emarefa.net/detail/BIM-479363
Modern Language Association (MLA)
Negri, Donatella R. M.…[et al.]. Nonintegrating Lentiviral Vector-Based Vaccine Efficiently Induces Functional and Persistent CD8+ T Cell Responses in Mice. Journal of Biomedicine and Biotechnology No. 2010 (2010), pp.1-7.
https://search.emarefa.net/detail/BIM-479363
American Medical Association (AMA)
Negri, Donatella R. M.& Michelini, Zuleika& Baroncelli, Silvia& Spada, Massimo& Vendetti, Silvia& Bona, Roberta…[et al.]. Nonintegrating Lentiviral Vector-Based Vaccine Efficiently Induces Functional and Persistent CD8+ T Cell Responses in Mice. Journal of Biomedicine and Biotechnology. 2010. Vol. 2010, no. 2010, pp.1-7.
https://search.emarefa.net/detail/BIM-479363
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-479363