Ethanolic Extract of Propolis Augments TRAIL-Induced Apoptotic Death in Prostate Cancer Cells
Joint Authors
Szliszka, Ewelina
Paradysz, Andrzej
Czuba, Zenon P.
Krol, Wojciech
Bronikowska, Joanna
Mertas, Anna
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-06-07
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Prostate cancer is a commonly diagnosed cancer in men.
The ethanolic extract of propolis (EEP) and its phenolic compounds possess immunomodulatory, chemopreventive and antitumor effects.
Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL/APO2L) is a naturally occurring anticancer agent that preferentially induces apoptosis in cancer cells and is not toxic to normal cells.
We examined the cytotoxic and apoptotic effects of EEP and phenolic compounds isolated from propolis in combination with TRAIL on two prostate cancer cell lines, hormone-sensitivity LNCaP and hormone-refractory DU145.
The cytotoxicity was evaluated by MTT and LDH assays.
The apoptosis was determined using flow cytometry with annexin V-FITC/propidium iodide.
The prostate cancer cell lines were proved to be resistant to TRAIL-induced apoptosis.
Our study demonstrated that EEP and its components significantly sensitize to TRAIL-induced death in prostate cancer cells.
The percentage of the apoptotic cells after cotreatment with 50 μg mL−1 EEP and 100 ng mL−1 TRAIL increased to 74.9 ± 0.7% for LNCaP and 57.4 ± 0.7% for DU145 cells.
The strongest cytotoxic effect on LNCaP cells was exhibited by apigenin, kaempferid, galangin and caffeic acid phenylethyl ester (CAPE) in combination with TRAIL (53.51 ± 0.68–66.06 ± 0.62% death cells).
In this work, we showed that EEP markedly augmented TRAIL-mediated apoptosis in prostate cancer cells and suggested the significant role of propolis in chemoprevention of prostate cancer.
American Psychological Association (APA)
Szliszka, Ewelina& Czuba, Zenon P.& Bronikowska, Joanna& Mertas, Anna& Paradysz, Andrzej& Krol, Wojciech. 2011. Ethanolic Extract of Propolis Augments TRAIL-Induced Apoptotic Death in Prostate Cancer Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2011, no. 2011, pp.1-11.
https://search.emarefa.net/detail/BIM-479421
Modern Language Association (MLA)
Szliszka, Ewelina…[et al.]. Ethanolic Extract of Propolis Augments TRAIL-Induced Apoptotic Death in Prostate Cancer Cells. Evidence-Based Complementary and Alternative Medicine No. 2011 (2011), pp.1-11.
https://search.emarefa.net/detail/BIM-479421
American Medical Association (AMA)
Szliszka, Ewelina& Czuba, Zenon P.& Bronikowska, Joanna& Mertas, Anna& Paradysz, Andrzej& Krol, Wojciech. Ethanolic Extract of Propolis Augments TRAIL-Induced Apoptotic Death in Prostate Cancer Cells. Evidence-Based Complementary and Alternative Medicine. 2011. Vol. 2011, no. 2011, pp.1-11.
https://search.emarefa.net/detail/BIM-479421
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-479421