Imaging Early Steps of Sindbis Virus Infection by Total Internal Reflection Fluorescence Microscopy
Joint Authors
Liu, Yuechueng
Gu, Youling
Yang, Yuanzheng
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-11-24
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Sindbis virus (SINV) is an alphavirus that has a broad host range and has been widely used as a vector for recombinant gene transduction, DNA-based vaccine production, and oncolytic cancer therapy.
The mechanism of SINV entry into host cells has yet to be fully understood.
In this paper, we used single virus tracking under total internal reflection fluorescence microscopy (TIRFM) to investigate SINV attachment to cell surface.
Biotinylated viral particles were labeled with quantum dots, which retained viral viability and infectivity.
By time-lapse imaging, we showed that the SINV exhibited a heterogeneous dynamics on the surface of the host cells.
Analysis of SINV motility demonstrated a two-step attachment reaction.
Moreover, dual color TIRFM of GFP-Rab5 and SINV suggested that the virus was targeted to the early endosomes after endocytosis.
These findings demonstrate the utility of quantum dot labeling in studying the early steps and behavior of SINV infection.
American Psychological Association (APA)
Gu, Youling& Yang, Yuanzheng& Liu, Yuechueng. 2011. Imaging Early Steps of Sindbis Virus Infection by Total Internal Reflection Fluorescence Microscopy. Advances in Virology،Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-479422
Modern Language Association (MLA)
Gu, Youling…[et al.]. Imaging Early Steps of Sindbis Virus Infection by Total Internal Reflection Fluorescence Microscopy. Advances in Virology No. 2011 (2011), pp.1-7.
https://search.emarefa.net/detail/BIM-479422
American Medical Association (AMA)
Gu, Youling& Yang, Yuanzheng& Liu, Yuechueng. Imaging Early Steps of Sindbis Virus Infection by Total Internal Reflection Fluorescence Microscopy. Advances in Virology. 2011. Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-479422
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-479422