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Effect of Allium cepa L. on Lipopolysaccharide-Stimulated Osteoclast Precursor Cell Viability, Count, and Morphology Using 4′,6-Diamidino-2-phenylindole-Staining
Joint Authors
Figueiredo, Camila A.
Da Silva Velozo, Eudes
Nogueira-Filho, Getulio
Stavroullakis, Alexander
Brito, Carlos
Prakki, Anuradha
Oliveira, Tatiane
Source
International Journal of Cell Biology
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-08-03
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Allium cepa L.
is known to possess numerous pharmacological properties.
Our aim was to examine the in vitro effects of Allium cepa L.
extract (AcE) on Porphyromonas gingivalis LPS and Escherichia coli LPS-stimulated osteoclast precursor cells to determine cell viability to other future cell-based assays.
Osteoclast precursor cells (RAW 264.7) were stimulated by Pg LPS (1 μg/mL) and E.
coli LPS (1 μg/mL) in the presence or absence of different concentrations of AcE (10–1000 μg/mL) for 5 days at 37°C/5% CO2.
Resazurin reduction and total protein content assays were used to detect cell viability.
AcE did not affect cell viability.
Resazurin reduction assay showed that AcE, at up to 1000 μg/mL, did not significantly affect cell viability and cellular protein levels.
Additionally a caspase 3/7 luminescence assay was used to disclose apoptosis and there was no difference in apoptotic activity between tested groups and control group.
Fluorescence images stained by DAPI showed no alteration on the morphology and cell counts of LPS-stimulated osteoclast precursor cells with the use of AcE in all tested concentrations when compared to control.
These findings suggest that Allium cepa L.
extract could be used for in vitro studies on Porphyromonas gingivalis LPS and Escherichia coli LPS-stimulated osteoclast precursor cells.
American Psychological Association (APA)
Oliveira, Tatiane& Figueiredo, Camila A.& Brito, Carlos& Stavroullakis, Alexander& Prakki, Anuradha& Da Silva Velozo, Eudes…[et al.]. 2014. Effect of Allium cepa L. on Lipopolysaccharide-Stimulated Osteoclast Precursor Cell Viability, Count, and Morphology Using 4′,6-Diamidino-2-phenylindole-Staining. International Journal of Cell Biology،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-479456
Modern Language Association (MLA)
Oliveira, Tatiane…[et al.]. Effect of Allium cepa L. on Lipopolysaccharide-Stimulated Osteoclast Precursor Cell Viability, Count, and Morphology Using 4′,6-Diamidino-2-phenylindole-Staining. International Journal of Cell Biology No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-479456
American Medical Association (AMA)
Oliveira, Tatiane& Figueiredo, Camila A.& Brito, Carlos& Stavroullakis, Alexander& Prakki, Anuradha& Da Silva Velozo, Eudes…[et al.]. Effect of Allium cepa L. on Lipopolysaccharide-Stimulated Osteoclast Precursor Cell Viability, Count, and Morphology Using 4′,6-Diamidino-2-phenylindole-Staining. International Journal of Cell Biology. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-479456
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-479456