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Insulin Preconditioning Elevates p-Akt and Cardiac Contractility after Reperfusion in the Isolated Ischemic Rat Heart
Joint Authors
Schricker, Thomas
Sato, Hiroaki
Sato, Tamaki
Matsukawa, Takashi
Lattermann, Ralph
Fukushima, Hisashi
Carvalho, George
Oguchi, Takeshi
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-08-13
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Insulin induces cardioprotection partly via an antiapoptotic effect.
However, the optimal timing of insulin administration for the best quality cardioprotection remains unclear.
We tested the hypothesis that insulin administered prior to ischemia provides better cardioprotection than insulin administration after ischemia.
Isolated rat hearts were prepared using Langendorff method and divided into three groups.
The Pre-Ins group (Pre-Ins) received 0.5 U/L insulin prior to 15 min no-flow ischemia for 20 min followed by 20 min of reperfusion.
The Post-Ins group (Post-Ins) received 0.5 U/L insulin during the reperfusion period only.
The control group (Control) was perfused with KH buffer throughout.
The maximum of left ventricular derivative of pressure development (dP/dt(max)) was recorded continuously.
Measurements of TNF-α and p-Akt in each time point were assayed by ELISA.
After reperfusion, dP/dt(max) in Pre-Ins was elevated, compared with Post-Ins at 10 minutes after reperfusion and Control at all-time points.
TNF-α levels at 5 minutes after reperfusion in the Pre-Ins were lower than the others.
After 5 minutes of reperfusion, p-Akt was elevated in Pre-Ins compared with the other groups.
Insulin administration prior to ischemia provides better cardioprotection than insulin administration only at reperfusion.
TNF-α suppression is possibly mediated via p-Akt leading to a reduction in contractile myocardial dysfunction.
American Psychological Association (APA)
Sato, Tamaki& Sato, Hiroaki& Oguchi, Takeshi& Fukushima, Hisashi& Carvalho, George& Lattermann, Ralph…[et al.]. 2014. Insulin Preconditioning Elevates p-Akt and Cardiac Contractility after Reperfusion in the Isolated Ischemic Rat Heart. BioMed Research International،Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-479535
Modern Language Association (MLA)
Sato, Tamaki…[et al.]. Insulin Preconditioning Elevates p-Akt and Cardiac Contractility after Reperfusion in the Isolated Ischemic Rat Heart. BioMed Research International No. 2014 (2014), pp.1-6.
https://search.emarefa.net/detail/BIM-479535
American Medical Association (AMA)
Sato, Tamaki& Sato, Hiroaki& Oguchi, Takeshi& Fukushima, Hisashi& Carvalho, George& Lattermann, Ralph…[et al.]. Insulin Preconditioning Elevates p-Akt and Cardiac Contractility after Reperfusion in the Isolated Ischemic Rat Heart. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-479535
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-479535