A Laccase with HIV-1 Reverse Transcriptase Inhibitory Activity from the Broth of Mycelial Culture of the Mushroom Lentinus tigrinus

Joint Authors

Xu, LiJing
Ng, Tzi Bun
Wang, He-Xiang

Source

Journal of Biomedicine and Biotechnology

Issue

Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-7, 7 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2012-03-21

Country of Publication

Egypt

No. of Pages

7

Main Subjects

Medicine

Abstract EN

A 59 kDa laccase with inhibitory activity against HIV-1 reverse transcriptase (IC50=2.4 μM) was isolated from the broth of mycelial culture of the mushroom Lentinus tigrinus.

The isolation procedure involved ion exchange chromatography on DEAE-cellulose and CM-cellulose, and gel filtration by fast protein liquid chromatography on Superdex 75.

The laccase was adsorbed on both types of ion exchangers.

About 95-fold purification was achieved with a 25.9% yield of the enzyme.

The procedure resulted in a specific enzyme activity of 76.6 U/mg.

Its N-terminal amino acid sequence was GIPDLHDLTV, which showed little similarity to other mushroom laccase and other Lentinus tigrinus strain laccase.

Its characteristics were different from previously reported laccase of other Lentinus tigrinus strain.

Maximal laccase activity was observed at a pH of 4 and at a temperature of 60°C, respectively.

This study yielded the information about the potentially exploitable activities of Lentinus tigrinus laccase.

American Psychological Association (APA)

Xu, LiJing& Wang, He-Xiang& Ng, Tzi Bun. 2012. A Laccase with HIV-1 Reverse Transcriptase Inhibitory Activity from the Broth of Mycelial Culture of the Mushroom Lentinus tigrinus. Journal of Biomedicine and Biotechnology،Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-479549

Modern Language Association (MLA)

Xu, LiJing…[et al.]. A Laccase with HIV-1 Reverse Transcriptase Inhibitory Activity from the Broth of Mycelial Culture of the Mushroom Lentinus tigrinus. Journal of Biomedicine and Biotechnology No. 2012 (2012), pp.1-7.
https://search.emarefa.net/detail/BIM-479549

American Medical Association (AMA)

Xu, LiJing& Wang, He-Xiang& Ng, Tzi Bun. A Laccase with HIV-1 Reverse Transcriptase Inhibitory Activity from the Broth of Mycelial Culture of the Mushroom Lentinus tigrinus. Journal of Biomedicine and Biotechnology. 2012. Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-479549

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-479549