Brain Activation by Peptide Pro-Leu-Gly-NH2 (MIF-1)
Joint Authors
Ehrensing, Rudolph H.
Yu, Chuanhui
He, Yi
Pan, Weihong
Kastin, Abba J.
Hsuchou, Hung
Stone, Kirsten Prufer
Khan, Reas S.
Source
International Journal of Peptides
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-03-28
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
MIF-1 (Pro-Leu-Gly-NH2) is a tripeptide for which the therapeutic potential in Parkinson's disease and depression has been indicated by many studies.
However, the cellular mechanisms of action of MIF-1 are not yet clear.
Here, we show the specific brain regions responsive to MIF-1 treatment by c-Fos mapping, and determine the kinetics of cellular signaling by western blotting of pERK, pSTAT3, and c-Fos in cultured neurons.
The immunoreactivity of c-Fos was increased 4 hours after MIF-1 treatment in brain regions critically involved in the regulation of mood, anxiety, depression, and memory.
The number of cells activated was greater after peripheral treatment (intravenous delivery) than after intracerebroventricular injection.
In cultured SH-SY5Y neuronal cells, c-Fos was induced time- and dose-dependently.
The activation of cellular c-Fos was preceded by a transient increase of mitogen-activated protein kinase pERK but a reduction of phosphorylated Signal Transducer and Activator of Transcription (pSTAT3) initially.
We conclude that MIF-1 can modulate multiple cellular signals including pERK, and pSTAT3 to activate c-Fos.
The cellular activation in specific brain regions illustrates the biochemical and neuroanatomical basis underlying the therapeutic effect of MIF-1 in Parkinson's disease and depression.
American Psychological Association (APA)
Khan, Reas S.& Yu, Chuanhui& Kastin, Abba J.& He, Yi& Ehrensing, Rudolph H.& Hsuchou, Hung…[et al.]. 2010. Brain Activation by Peptide Pro-Leu-Gly-NH2 (MIF-1). International Journal of Peptides،Vol. 2010, no. 2010, pp.1-10.
https://search.emarefa.net/detail/BIM-479640
Modern Language Association (MLA)
Khan, Reas S.…[et al.]. Brain Activation by Peptide Pro-Leu-Gly-NH2 (MIF-1). International Journal of Peptides No. 2010 (2010), pp.1-10.
https://search.emarefa.net/detail/BIM-479640
American Medical Association (AMA)
Khan, Reas S.& Yu, Chuanhui& Kastin, Abba J.& He, Yi& Ehrensing, Rudolph H.& Hsuchou, Hung…[et al.]. Brain Activation by Peptide Pro-Leu-Gly-NH2 (MIF-1). International Journal of Peptides. 2010. Vol. 2010, no. 2010, pp.1-10.
https://search.emarefa.net/detail/BIM-479640
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-479640