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Target Capture and Massive Sequencing of Genes Transcribed in Mytilus galloprovincialis
Joint Authors
Rosani, Umberto
Venier, Paola
Domeneghetti, Stefania
Pallavicini, Alberto
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-06-30
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Next generation sequencing (NGS) allows fast and massive production of both genome and transcriptome sequence datasets.
As the genome of the Mediterranean mussel Mytilus galloprovincialis is not available at present, we have explored the possibility of reducing the whole genome sequencing efforts by using capture probes coupled with PCR amplification and high-throughput 454-sequencing to enrich selected genomic regions.
The enrichment of DNA target sequences was validated by real-time PCR, whereas the efficacy of the applied strategy was evaluated by mapping the 454-output reads against reference transcript data already available for M.
galloprovincialis and by measuring coverage, SNPs, number of de novo sequenced introns, and complete gene sequences.
Focusing on a target size of nearly 1.5 Mbp, we obtained a target coverage which allowed the identification of more than 250 complete introns, 10,741 SNPs, and also complete gene sequences.
This study confirms the transcriptome-based enrichment of gDNA regions as a good strategy to expand knowledge on specific subsets of genes also in nonmodel organisms.
American Psychological Association (APA)
Rosani, Umberto& Domeneghetti, Stefania& Pallavicini, Alberto& Venier, Paola. 2014. Target Capture and Massive Sequencing of Genes Transcribed in Mytilus galloprovincialis. BioMed Research International،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-479716
Modern Language Association (MLA)
Rosani, Umberto…[et al.]. Target Capture and Massive Sequencing of Genes Transcribed in Mytilus galloprovincialis. BioMed Research International No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-479716
American Medical Association (AMA)
Rosani, Umberto& Domeneghetti, Stefania& Pallavicini, Alberto& Venier, Paola. Target Capture and Massive Sequencing of Genes Transcribed in Mytilus galloprovincialis. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-479716
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-479716