![](/images/graphics-bg.png)
Plasma Albumin Induces Cytosolic Calcium Oscilations and DNA Synthesis in Human Cultured Astrocytes
Joint Authors
Vega-Zelaya, Lorena
Ortega, Guillermo J.
Sola, Rafael G.
Pastor, Jesús
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-22
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
So far, a little is known about transition from normal to focal epileptic brain, although disruption in blood-brain barrier and albumin had recently involved.
The main objective of this work is to characterize the response of cultured human astrocytes to plasma albumin, including induction of DNA synthesis.
Cortical tissue was obtained from 9 patients operated from temporal lobe epilepsy.
Astrocytes were cultured for 3-4 weeks and cytosolic calcium concentration (Ca2+c) was measured.
Bovine and human plasma albumin were used.
We observed that low albumin concentration decreases Ca2+c, while higher concentration, induces increase in Ca2+c.
It was shown that increase in Ca2+c was mediated by inositol 1,4,5-trisphosphate and released from internal stores.
Increase in Ca2+c was reduced to 19% by blocking the transforming growth factor-beta (TGF-βR) receptor.
Albumin induces DNA synthesis in a dose-response manner.
Finally, induction of DNA synthesis can be partially blocked by heparin and block of TGF-β; however, the combination of both incompletely inhibits DNA synthesis.
Therefore, results suggest that mechanisms other than Ca2+ signals and TGF-β receptor activation might induce DNA synthesis in a lesser degree.
These results may be important to further understand the mechanisms involved in the transition from normal to focal epileptic brain.
American Psychological Association (APA)
Vega-Zelaya, Lorena& Ortega, Guillermo J.& Sola, Rafael G.& Pastor, Jesús. 2014. Plasma Albumin Induces Cytosolic Calcium Oscilations and DNA Synthesis in Human Cultured Astrocytes. BioMed Research International،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-479765
Modern Language Association (MLA)
Vega-Zelaya, Lorena…[et al.]. Plasma Albumin Induces Cytosolic Calcium Oscilations and DNA Synthesis in Human Cultured Astrocytes. BioMed Research International No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-479765
American Medical Association (AMA)
Vega-Zelaya, Lorena& Ortega, Guillermo J.& Sola, Rafael G.& Pastor, Jesús. Plasma Albumin Induces Cytosolic Calcium Oscilations and DNA Synthesis in Human Cultured Astrocytes. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-479765
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-479765