Screening for Y Chromosome Microdeletion in a Nonobstructive Azoospermic Male Patient with Allogeneic Bone Marrow Transplantation from His Sister
Joint Authors
Kadioglu, Ates
Aydın, Filiz
Gurkan, Hakan
Akman, Tolga
Kucukdurmaz, Faruk
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-4, 4 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-12-16
Country of Publication
Egypt
No. of Pages
4
Main Subjects
Abstract EN
Genomic DNA of a patient diagnosed with nonobstructive azoospermia and with the history of allogenic bone marrow transplantation from his sister due to chronic myeloid leukemia was isolated from peripheral blood in order to screen Y chromosome microdeletions.
13 short tagged sites belonging to AZF a, b, and c loci were detected with multiplex polymerase chain reaction technique.
Bands were determined in ZFX/ZFY wells, whereas no bands were determined in wells of other STS regions.
DNA isolation was done from buccal mucosa smear to obtain genomic DNA from patient's own cells and multiplex polymerase chain reaction technique was performed again.
Bands were seen in all wells of 13 STS regions.
Y chromosome microdeletion was not detected in the patient.
In conclusion, genomic DNA isolation in patients undergoing BMT should be done from patients' own cells.
American Psychological Association (APA)
Gurkan, Hakan& Kucukdurmaz, Faruk& Akman, Tolga& Aydın, Filiz& Kadioglu, Ates. 2010. Screening for Y Chromosome Microdeletion in a Nonobstructive Azoospermic Male Patient with Allogeneic Bone Marrow Transplantation from His Sister. Case Reports in Medicine،Vol. 2010, no. 2010, pp.1-4.
https://search.emarefa.net/detail/BIM-479957
Modern Language Association (MLA)
Gurkan, Hakan…[et al.]. Screening for Y Chromosome Microdeletion in a Nonobstructive Azoospermic Male Patient with Allogeneic Bone Marrow Transplantation from His Sister. Case Reports in Medicine No. 2010 (2010), pp.1-4.
https://search.emarefa.net/detail/BIM-479957
American Medical Association (AMA)
Gurkan, Hakan& Kucukdurmaz, Faruk& Akman, Tolga& Aydın, Filiz& Kadioglu, Ates. Screening for Y Chromosome Microdeletion in a Nonobstructive Azoospermic Male Patient with Allogeneic Bone Marrow Transplantation from His Sister. Case Reports in Medicine. 2010. Vol. 2010, no. 2010, pp.1-4.
https://search.emarefa.net/detail/BIM-479957
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-479957