In Vitro Study on Apoptosis Induced by Strontium-89 in Human Breast Carcinoma Cell Line
Joint Authors
Zhu, Min
Wang, Jing
Bao, Weiguang
Jiang, Han
Wang, Cheng
Chen, Baoguo
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-06-09
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Many radiopharmaceuticals used for medical diagnosis and therapy are beta emitters; however, the mechanism of the cell death caused by beta-irradiation is not well understood.
The objective of this study was to investigate the apoptosis of human breast carcinoma MCF-7 cell lines induced by Strontium-89 (89Sr) and its regulation and control mechanism.
High-metastatic Breast Carcinoma MCF-7 cells were cultured in vitro using 89Sr with different radioactive concentration.
The inhibition rate of cell proliferation was measured by MTT color matching method.
The cell cycle retardation, apoptosis conditions, mitochondrion transmembrane potential difference and Fas expression were tested and analyzed.
The genes P53 and bcl-2 expressions was also analyzed using immunity histochemical analysis.
After being induced by 89Sr with various of radioactive concentration, it was found that the inhibition of cell proliferation of MCF-7 cells was obviously, the retardation of cell cycle occurred mainly in G2-M.
It was also found that the obvious apoptosis occurred after being induced by 89Sr, the highest apoptosis rate reached 46.28%.
The expressions of Fas acceptor and P53 gene increased, while bcl-2 gene expression decreasesd.
These findings demonstrate that in the ranges of a certain radioactive concentration, the inhibition rate of MCF-7 cell proliferation and retardation of cell cycle had positive correlation with the concentration of 89Sr.
And the mitochondrion transmembrane potential decrease would induce the apoptosis of MCF-7 cell notably, which were controlled by P53 and bcl-2 genes, involved with the Fas acceptor.
American Psychological Association (APA)
Wang, Cheng& Wang, Jing& Jiang, Han& Zhu, Min& Chen, Baoguo& Bao, Weiguang. 2011. In Vitro Study on Apoptosis Induced by Strontium-89 in Human Breast Carcinoma Cell Line. Journal of Biomedicine and Biotechnology،Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-480001
Modern Language Association (MLA)
Wang, Cheng…[et al.]. In Vitro Study on Apoptosis Induced by Strontium-89 in Human Breast Carcinoma Cell Line. Journal of Biomedicine and Biotechnology No. 2011 (2011), pp.1-7.
https://search.emarefa.net/detail/BIM-480001
American Medical Association (AMA)
Wang, Cheng& Wang, Jing& Jiang, Han& Zhu, Min& Chen, Baoguo& Bao, Weiguang. In Vitro Study on Apoptosis Induced by Strontium-89 in Human Breast Carcinoma Cell Line. Journal of Biomedicine and Biotechnology. 2011. Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-480001
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-480001