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Individual Rac GTPases Mediate Aspects of Prostate Cancer Cell and Bone Marrow Endothelial Cell Interactions
Joint Authors
Chatterjee, Moumita
van Golen, Kenneth L.
Jenkins-Kabaila, Mashariki
Dubyk, Cara W.
Sequeira, Linda
Pathak, Surabhi
Source
Journal of Signal Transduction
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-06-27
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
The Rho GTPases organize the actin cytoskeleton and are involved in cancer metastasis.
Previously, we demonstrated that RhoC GTPase was required for PC-3 prostate cancer cell invasion.
Targeted down-regulation of RhoC led to sustained activation of Rac1 GTPase and morphological, molecular and phenotypic changes reminiscent of epithelial to mesenchymal transition.
We also reported that Rac1 is required for PC-3 cell diapedesis across a bone marrow endothelial cell layer.
In the current study, we queried whether Rac3 and RhoG GTPases also have a role in prostate tumor cell diapedesis.
Using specific siRNAs we demonstrate roles for each protein in PC-3 and C4-2 cell adhesion and diapedesis.
We have shown that the chemokine CCL2 induces tumor cell diapedesis via Rac1 activation.
Here we find that RhoG partially contributes to CCL2-induced tumor cell diapedesis.
We also find that Rac1 GTPase mediates tight binding of prostate cancer cells to bone marrow endothelial cells and promotes retraction of endothelial cells required for tumor cell diapedesis.
Finally, Rac1 leads to β1 integrin activation, suggesting a mechanism that Rac1 can mediate tight binding with endothelial cells.
Together, our data suggest that Rac1 GTPase is key mediator of prostate cancer cell-bone marrow endothelial cell interactions.
American Psychological Association (APA)
Chatterjee, Moumita& Sequeira, Linda& Jenkins-Kabaila, Mashariki& Dubyk, Cara W.& Pathak, Surabhi& van Golen, Kenneth L.. 2011. Individual Rac GTPases Mediate Aspects of Prostate Cancer Cell and Bone Marrow Endothelial Cell Interactions. Journal of Signal Transduction،Vol. 2011, no. 2011, pp.1-13.
https://search.emarefa.net/detail/BIM-480046
Modern Language Association (MLA)
Chatterjee, Moumita…[et al.]. Individual Rac GTPases Mediate Aspects of Prostate Cancer Cell and Bone Marrow Endothelial Cell Interactions. Journal of Signal Transduction No. 2011 (2011), pp.1-13.
https://search.emarefa.net/detail/BIM-480046
American Medical Association (AMA)
Chatterjee, Moumita& Sequeira, Linda& Jenkins-Kabaila, Mashariki& Dubyk, Cara W.& Pathak, Surabhi& van Golen, Kenneth L.. Individual Rac GTPases Mediate Aspects of Prostate Cancer Cell and Bone Marrow Endothelial Cell Interactions. Journal of Signal Transduction. 2011. Vol. 2011, no. 2011, pp.1-13.
https://search.emarefa.net/detail/BIM-480046
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-480046