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Association of TCTP with Centrosome and Microtubules
Joint Authors
Chesnel, Franck
Bazile, Franck
Kubiak, Jacek Z.
Jaglarz, Mariusz K.
Laskowska, Katarzyna
Kloc, Malgorzata
Borsuk, Ewa
Polanski, Zbigniew
Source
Biochemistry Research International
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-05-14
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Translationally Controlled Tumour Protein (TCTP) associates with microtubules (MT), however, the details of this association are unknown.
Here we analyze the relationship of TCTP with MTs and centrosomes in Xenopus laevis and mammalian cells using immunofluorescence, tagged TCTP expression and immunoelectron microscopy.
We show that TCTP associates both with MTs and centrosomes at spindle poles when detected by species-specific antibodies and by Myc-XlTCTP expression in Xenopus and mammalian cells.
However, when the antibodies against XlTCTP were used in mammalian cells, TCTP was detected exclusively in the centrosomes.
These results suggest that a distinct pool of TCTP may be specific for, and associate with, the centrosomes.
Double labelling for TCTP and γ-tubulin with immuno-gold electron microscopy in Xenopus laevis oogonia shows localization of TCTP at the periphery of the γ-tubulin-containing pericentriolar material (PCM) enveloping the centriole.
TCTP localizes in the close vicinity of, but not directly on the MTs in Xenopus ovary suggesting that this association requires unidentified linker proteins.
Thus, we show for the first time: (1) the association of TCTP with centrosomes, (2) peripheral localization of TCTP in relation to the centriole and the γ-tubulin-containing PCM within the centrosome, and (3) the indirect association of TCTP with MTs.
American Psychological Association (APA)
Jaglarz, Mariusz K.& Bazile, Franck& Laskowska, Katarzyna& Polanski, Zbigniew& Chesnel, Franck& Borsuk, Ewa…[et al.]. 2012. Association of TCTP with Centrosome and Microtubules. Biochemistry Research International،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-480054
Modern Language Association (MLA)
Jaglarz, Mariusz K.…[et al.]. Association of TCTP with Centrosome and Microtubules. Biochemistry Research International No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-480054
American Medical Association (AMA)
Jaglarz, Mariusz K.& Bazile, Franck& Laskowska, Katarzyna& Polanski, Zbigniew& Chesnel, Franck& Borsuk, Ewa…[et al.]. Association of TCTP with Centrosome and Microtubules. Biochemistry Research International. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-480054
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-480054