Novel Simplified and Rapid Method for Screening and Isolation of Polyunsaturated Fatty Acids Producing Marine Bacteria
Joint Authors
Source
Biotechnology Research International
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-08-15
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Information Technology and Computer Science
Abstract EN
Bacterial production of polyunsaturated fatty acids (PUFAs) is a potential biotechnological approach for production of valuable nutraceuticals.
Reliable method for screening of number of strains within short period of time is great need.
Here, we report a novel simplified method for screening and isolation of PUFA-producing bacteria by direct visualization using the H2O2-plate assay.
The oxidative stability of PUFAs in growing bacteria towards added H2O2 is a distinguishing characteristic between the PUFAs producers (no zone of inhibition) and non-PUFAs producers (zone of inhibition) by direct visualization.
The confirmation of assay results was performed by injecting fatty acid methyl esters (FAMEs) produced by selected marine bacteria to Gas Chromatography-Mass Spectrometry (GCMS).
To date, this assay is the most effective, inexpensive, and specific method for bacteria producing PUFAs and shows drastically reduction in the number of samples thus saves the time, effort, and cost of screening and isolating strains of bacterial PUFAs producers.
American Psychological Association (APA)
Tilay, Ashwini& Annapure, Uday. 2012. Novel Simplified and Rapid Method for Screening and Isolation of Polyunsaturated Fatty Acids Producing Marine Bacteria. Biotechnology Research International،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-480126
Modern Language Association (MLA)
Tilay, Ashwini& Annapure, Uday. Novel Simplified and Rapid Method for Screening and Isolation of Polyunsaturated Fatty Acids Producing Marine Bacteria. Biotechnology Research International No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-480126
American Medical Association (AMA)
Tilay, Ashwini& Annapure, Uday. Novel Simplified and Rapid Method for Screening and Isolation of Polyunsaturated Fatty Acids Producing Marine Bacteria. Biotechnology Research International. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-480126
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-480126