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Detection of Streptococcus mutans Genomic DNA in Human DNA Samples Extracted from Saliva and Blood
Joint Authors
Rozhon, Christopher
Seymen, Figen
Schulhof, Louise P.
Brandon, Carla A.
Letra, Ariadne
Castilla, Eduardo E.
Noel, Jacqueline B.
McNeil, Daniel W.
Resick, Judith M.
DeSensi, Rebecca S.
Mereb, Juan C.
Anjomshoaa, Ida
Yildirim, Mine
Marazita, Mary L.
Vieira, Alexandre Rezende
Deeley, Kathleen B.
Gandhi, Pooja
Menezes, Renato
Carricato, Wendy M.
Weyant, Robert J.
Poletta, Fernando A.
Lopez-Camelo, Jorge S.
Callahan, Nicholas F.
Modesto Vieira, Adriana
Crout, Richard
Orioli, Iêda M.
Wendell, Steven
Patir, Asli
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-04-10
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Caries is a multifactorial disease, and studies aiming to unravel the factors modulating its etiology must consider all known predisposing factors.
One major factor is bacterial colonization, and Streptococcus mutans is the main microorganism associated with the initiation of the disease.
In our studies, we have access to DNA samples extracted from human saliva and blood.
In this report, we tested a real-time PCR assay developed to detect copies of genomic DNA from Streptococcus mutans in 1,424 DNA samples from humans.
Our results suggest that we can determine the presence of genomic DNA copies of Streptococcus mutans in both DNA samples from caries-free and caries-affected individuals.
However, we were not able to detect the presence of genomic DNA copies of Streptococcus mutans in any DNA samples extracted from peripheral blood, which suggests the assay may not be sensitive enough for this goal.
Values of the threshold cycle of the real-time PCR reaction correlate with higher levels of caries experience in children, but this correlation could not be detected for adults.
American Psychological Association (APA)
Vieira, Alexandre Rezende& Deeley, Kathleen B.& Callahan, Nicholas F.& Noel, Jacqueline B.& Anjomshoaa, Ida& Carricato, Wendy M.…[et al.]. 2011. Detection of Streptococcus mutans Genomic DNA in Human DNA Samples Extracted from Saliva and Blood. ISRN Dentistry،Vol. 2011, no. 2011, pp.1-6.
https://search.emarefa.net/detail/BIM-480217
Modern Language Association (MLA)
Vieira, Alexandre Rezende…[et al.]. Detection of Streptococcus mutans Genomic DNA in Human DNA Samples Extracted from Saliva and Blood. ISRN Dentistry No. 2011 (2011), pp.1-6.
https://search.emarefa.net/detail/BIM-480217
American Medical Association (AMA)
Vieira, Alexandre Rezende& Deeley, Kathleen B.& Callahan, Nicholas F.& Noel, Jacqueline B.& Anjomshoaa, Ida& Carricato, Wendy M.…[et al.]. Detection of Streptococcus mutans Genomic DNA in Human DNA Samples Extracted from Saliva and Blood. ISRN Dentistry. 2011. Vol. 2011, no. 2011, pp.1-6.
https://search.emarefa.net/detail/BIM-480217
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-480217