Multicomponent Therapeutics of Berberine Alkaloids
Joint Authors
Xiao, Xiaohe
Dong, Xiaoping
Yang, Meihua
Yan, Dan
Luo, Jiaoyang
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-03-24
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Although berberine alkaloids (BAs) are reported to be with broad-spectrum antibacterial and antiviral activities, the interactions among BAs have not been elucidated.
In the present study, methicillin-resistant Staphylococcus aureus (MRSA) was chosen as a model organism, and modified broth microdilution was applied for the determination of the fluorescence absorption values to calculate the anti-MRSA activity of BAs.
We have initiated four steps to seek the optimal combination of BAs that are (1) determining the anti-MRSA activity of single BA, (2) investigating the two-component combination to clarify the interactions among BAs by checkerboard assay, (3) investigating the multicomponent combination to determine the optimal ratio by quadratic rotation-orthogonal combination design, and (4) in vivo and in vitro validation of the optimal combination.
The results showed that the interactions among BAs are related to their concentrations.
The synergetic combinations included “berberine and epiberberine,” “jatrorrhizine and palmatine” and “jatrorrhizine and coptisine”; the antagonistic combinations included “coptisine and epiberberine”.
The optimal combination was berberine : coptisine : jatrorrhizine : palmatine : epiberberine = 0.702 : 0.863 : 1 : 0.491 : 0.526, and the potency of the optimal combination on cyclophosphamide-immunocompromised mouse model was better than the natural combinations of herbs containing BAs.
American Psychological Association (APA)
Luo, Jiaoyang& Yan, Dan& Yang, Meihua& Dong, Xiaoping& Xiao, Xiaohe. 2013. Multicomponent Therapeutics of Berberine Alkaloids. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-480327
Modern Language Association (MLA)
Luo, Jiaoyang…[et al.]. Multicomponent Therapeutics of Berberine Alkaloids. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-480327
American Medical Association (AMA)
Luo, Jiaoyang& Yan, Dan& Yang, Meihua& Dong, Xiaoping& Xiao, Xiaohe. Multicomponent Therapeutics of Berberine Alkaloids. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-480327
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-480327