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PPAR-γ Impairment Alters Peroxisome Functionality in Primary Astrocyte Cell Cultures
Joint Authors
Ghelardini, Carla
Micheli, Laura
Zanardelli, Matteo
Di Cesare Mannelli, Lorenzo
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-03-04
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Peroxisomes provide glial cells with protective functions against the harmful effects of H2O2 on neurons and peroxisome impairment results in nervous lesions.
Agonists of the γ-subtype of the Peroxisome-Proliferator-Activated-Receptors (PPAR) have been proposed as neuroprotective agents in neurodegenerative disorders.
Nevertheless, the role of PPAR-γ alterations in pathophysiological mechanisms and the relevance of peroxisome functions in the PPAR-γ effects are not yet clear.
In a primary cell culture of rat astrocytes, the irreversible PPAR-γ antagonist GW9662 concentration-dependently decreased the activity of catalase, the most important antioxidant defense enzyme in peroxisomes.
Catalase functionality recovered in a few days and the PPAR-γ agonist rosiglitazone promoted reversal of enzymatic damage.
The reversible antagonist G3335 reduced both the activity and expression of catalase in a rosiglitazone-prevented manner.
G3335 reduced also the glutathione reductase expression, indicating that enzyme involved in glutathione regeneration was compromised.
Neither the PPAR-α target gene Acyl-Coenzyme-A-oxidase-1 nor the mitochondrial detoxifying enzyme NADH:ubiquinone-oxidoreductase (NDFUS3) was altered by PPAR-γ inhibition.
In conclusion, PPAR-γ inhibition induced impairment of catalase in astrocytes.
A general decrease of the antioxidant defenses of the cell suggests that a PPAR-γ hypofunction could participate in neurodegenerative mechanisms through peroxisomal damage.
This series of experiments could be a useful model for studying compounds able to restore peroxisome functionality.
American Psychological Association (APA)
Di Cesare Mannelli, Lorenzo& Zanardelli, Matteo& Micheli, Laura& Ghelardini, Carla. 2014. PPAR-γ Impairment Alters Peroxisome Functionality in Primary Astrocyte Cell Cultures. BioMed Research International،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-480378
Modern Language Association (MLA)
Di Cesare Mannelli, Lorenzo…[et al.]. PPAR-γ Impairment Alters Peroxisome Functionality in Primary Astrocyte Cell Cultures. BioMed Research International No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-480378
American Medical Association (AMA)
Di Cesare Mannelli, Lorenzo& Zanardelli, Matteo& Micheli, Laura& Ghelardini, Carla. PPAR-γ Impairment Alters Peroxisome Functionality in Primary Astrocyte Cell Cultures. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-480378
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-480378