Antiangiogenesis Therapy of Endometriosis Using PAMAM as a Gene Vector in a Noninvasive Animal Model
Joint Authors
Wang, Liantang
Liang, Lili
Tan, Jinfeng
Wu, Yanxin
Zhan, Xuejun
Xie, Hongzhe
Ke, Peiqi
Liu, Bin
Huang, Jiaming
Guo, Xu
Wang, Ningning
Liu, Yongdong
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-06-24
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Objective.
To evaluate the characteristics and antiangiogenic effects of endostatin-loaded PAMAM on endometriosis in a noninvasive animal model.
Materials and Methods.
A noninvasive animal model was established by injecting adenovirus-GFP transfected endometrial stromal and glandular epithelial cells subcutaneously into nude mice.
Endostatin-loaded PAMAM was prepared and identified by transmission electron microscopy.
For in vitro studies, the DNA protection and cytotoxicity of PAMAM were investigated and compared with Lipofectamine 2000.
For in vivo study, endostatin-loaded PAMAM was injected into the noninvasive model and evaluated by continuously observing the fluorescent lesion, lesion weight, microvessel density and VEGF immunostaining.
Results.
Compared with Lipofectamine 2000, PAMAM and HC PAMAM-ES group, MC PAMAM-ES group and LC PAMAM-ES group demonstrated a better stromal cells protective such that MC PAMAM-ES group of CCK8 was 0.617 ± 0.122 at 24 hr and 0.668 ± 0.143 at 48 hr and LC PAMAM-ES group of CCK8 was 0.499 ± 0.103 at 24 hr and 0.610 ± 0.080 at 48 hr in stromal cells (P<0.05) but similar cytotoxicity in glandular epithelial cells in vitro.
After 16 hrs of digestion, DNA decreased slightly under the protection of PAMAM.
Endostatin-loaded PAMAM of HD PAMAM-ES group and LD PAMAM-ES group inhibited the growth of the endometriotic lesion in vivo at days 15, 20, 25 and 30 detected by noninvasive observation after injecting one dose endostatin of various medicines into the endometrial lesion in each mouse on day 10 (P<0.05) and confirmed by lesion weight at day 30 with HD PAMAM-ES group being 0.0104 ± 0.0077 g and LD PAMAM-ES group being 0.0140 ± 0.0097 g (P<0.05).
Immunohistochemistry results showed that endostatin-loaded PAMAM reduced the microvessel density 3.8 ± 2.4 especially in HD PAMAM-ES group in the lesion (P<0.05).
Conclusion.
Endostatin-loaded PAMAM inhibits the development of endometriosis through an antiangiogenic mechanism and can be observed through the noninvasive endometriosis model.
American Psychological Association (APA)
Wang, Ningning& Liu, Bin& Liang, Lili& Wu, Yanxin& Xie, Hongzhe& Huang, Jiaming…[et al.]. 2014. Antiangiogenesis Therapy of Endometriosis Using PAMAM as a Gene Vector in a Noninvasive Animal Model. BioMed Research International،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-480382
Modern Language Association (MLA)
Wang, Ningning…[et al.]. Antiangiogenesis Therapy of Endometriosis Using PAMAM as a Gene Vector in a Noninvasive Animal Model. BioMed Research International No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-480382
American Medical Association (AMA)
Wang, Ningning& Liu, Bin& Liang, Lili& Wu, Yanxin& Xie, Hongzhe& Huang, Jiaming…[et al.]. Antiangiogenesis Therapy of Endometriosis Using PAMAM as a Gene Vector in a Noninvasive Animal Model. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-480382
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-480382