Protective Effects of Peroxisome Proliferator-Activated Receptor-α Agonist, Wy14643, on HypoxiaReoxygenation Injury in Primary Rat Hepatocytes

Abstract EN

This study investigates the effects and possible mechanism of an agonist of PPARα, Wy14643, on primary hepatocytes subjected to H/R injury in rats.

H/R induced a significant increase ALT, AST, MDA in the culture medium and ROS in the hepatocytes.

These effects were reversed by pretreatment with Wy14643 in the dose-dependent manner.

The activity of SOD and the level of GSH in the hepatocytes were decreased after H/R, which were increased by Wy14643 pretreatment.

Moreover, the mRNA expressions of PPARα significantly increased in H/R+Wy14643 groups when compared with that in H/R group.

A PPARα agonist, Wy14643, exerts significant protective effect against H/R injury in primary hepatocytes via PPARα activation and attenuating oxidative stress.