IL-21 Modulates Release of Proinflammatory Cytokines in LPS-Stimulated Macrophages through Distinct Signaling Pathways
Joint Authors
Liu, Bing-run
Chen, Wei
Liu, Ju-xiong
Lv, Qing-kang
Wang, Wei
Li, Yang
Fu, Shou-peng
Wang, Jian-fa
Xue, Wen-jing
Huang, Bing-xu
Li, Zhi-qiang
Xie, Shan-shan
Liu, Hong-mei
Li, Su-nan
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-12-26
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
The aim of this study was to investigate the anti-inflammatory effect of IL-21 on LPS-induced mouse peritoneal macrophages.
The results showed that IL-21 significantly inhibited LPS-induced mRNA expression of IL-1β, TNF-α, and IL-6 in macrophages, but not of IFN-γ, IL-10, CCL5, or CXCL2.
ELISA analysis showed that IL-21 also suppressed LPS-induced production of TNF-α and IL-6 in culture supernatants.
Western blot analysis showed that IL-21 clearly inhibited ERK and IκBα phosphorylation and NF-κB translocation in LPS-stimulated macrophages, but it increased STAT3 phosphorylation.
Flow cytometric and Western blot analysis showed that IL-21 decreased M1 macrophages surface markers expression of CD86, iNOS, and TLR4 in LPS-stimulated cells.
All results suggested that IL-21 decreases IL-6 and TNF-α production via inhibiting the phosphorylation of ERK and translocation of NF-κB and promotes a shift from the M1 to M2 macrophage phenotype by decreasing the expression of CD86, iNOS, and TLR4 and by increasing STAT3 phosphorylation in LPS-stimulated cells.
American Psychological Association (APA)
Li, Su-nan& Wang, Wei& Fu, Shou-peng& Wang, Jian-fa& Liu, Hong-mei& Xie, Shan-shan…[et al.]. 2013. IL-21 Modulates Release of Proinflammatory Cytokines in LPS-Stimulated Macrophages through Distinct Signaling Pathways. Mediators of Inflammation،Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-480512
Modern Language Association (MLA)
Li, Su-nan…[et al.]. IL-21 Modulates Release of Proinflammatory Cytokines in LPS-Stimulated Macrophages through Distinct Signaling Pathways. Mediators of Inflammation No. 2013 (2013), pp.1-12.
https://search.emarefa.net/detail/BIM-480512
American Medical Association (AMA)
Li, Su-nan& Wang, Wei& Fu, Shou-peng& Wang, Jian-fa& Liu, Hong-mei& Xie, Shan-shan…[et al.]. IL-21 Modulates Release of Proinflammatory Cytokines in LPS-Stimulated Macrophages through Distinct Signaling Pathways. Mediators of Inflammation. 2013. Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-480512
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-480512