Single Layer Centrifugation with Androcoll-P Can Be Scaled-Up to Process Larger Volumes of Boar Semen
Joint Authors
Parlevliet, Joyce
Johannisson, Anders
Wallgren, Margareta
van Wienen, Marjet
Morrell, Jane M.
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-11-29
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
The objective of this study was to scale-up the procedure for Single Layer Centrifugation (SLC) through AndrocollTM-P, as a preliminary step towords processing the whole ejaculate.
The first experiment compared Single Layer Centrifugation using 4.5 mL and 15 mL extended ejaculate (SLC-4.5 and SLC-15, resp.), assessing sperm quality by objective motility analysis, morphology, viability, and the production of reactive oxygen species (ROS).
In the second experiment, SLC-4.5 was compared to Single Layer Centrifugation with 25 mL extended ejaculate (SLC-25) using motility analysis and morphology.
In both experiments, normal morphology and linear motility were significantly higher in the SLC-selected samples than in the uncentrifuged controls (P<.001), whereas total motility and membrane integrity were unchanged.
Although ROS production was higher in the SLC-selected samples than in the controls (P<.01), this might have been due to the presence of antioxidants in seminal plasma in the latter.
In conclusion, there was no difference in sperm quality between SLC-4.5 and SLC-15 samples, or between SLC-4.5 and SLC-25 samples, indicating that the SLC method can be scaled-up successfully.
American Psychological Association (APA)
van Wienen, Marjet& Johannisson, Anders& Wallgren, Margareta& Parlevliet, Joyce& Morrell, Jane M.. 2010. Single Layer Centrifugation with Androcoll-P Can Be Scaled-Up to Process Larger Volumes of Boar Semen. ISRN Veterinary Science،Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-480555
Modern Language Association (MLA)
van Wienen, Marjet…[et al.]. Single Layer Centrifugation with Androcoll-P Can Be Scaled-Up to Process Larger Volumes of Boar Semen. ISRN Veterinary Science No. 2011 (2011), pp.1-8.
https://search.emarefa.net/detail/BIM-480555
American Medical Association (AMA)
van Wienen, Marjet& Johannisson, Anders& Wallgren, Margareta& Parlevliet, Joyce& Morrell, Jane M.. Single Layer Centrifugation with Androcoll-P Can Be Scaled-Up to Process Larger Volumes of Boar Semen. ISRN Veterinary Science. 2010. Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-480555
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-480555