Identification and Partial Characterization of an L-Tyrosine Aminotransferase (TAT) from Arabidopsis thaliana
Joint Authors
Prabhu, Pranav R.
Hudson, André O.
Source
Biochemistry Research International
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-08-04
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
The aminotransferase gene family in the model plant Arabidopsis thaliana consists of 44 genes.
Twenty six of these enzymes are classified as characterized meaning that the reaction(s) that the enzyme catalyzes are documented using experimental means.
The remaining 18 enzymes are uncharacterized and are therefore deemed putative.
Our laboratory is interested in elucidating the function(s) of the remaining putative aminotransferase enzymes.
To this end, we have identified and partially characterized an aminotransferase (TAT) enzyme from Arabidopsis annotated by the locus tag At5g36160.
The full-length cDNA was cloned and the purified recombinant enzyme was characterized using in vitro and in vivo experiments.
In vitro analysis showed that the enzyme is capable of interconverting L-Tyrosine and 4-hydroxyphenylpyruvate, and L-Phenylalanine and phenylpyruvate.
In vivo analysis by functional complementation showed that the gene was able to complement an E.
coli with a background of aminotransferase mutations that confers auxotrophy for L-Tyrosine and L-Phenylalanine.
American Psychological Association (APA)
Prabhu, Pranav R.& Hudson, André O.. 2010. Identification and Partial Characterization of an L-Tyrosine Aminotransferase (TAT) from Arabidopsis thaliana. Biochemistry Research International،Vol. 2010, no. 2010, pp.1-11.
https://search.emarefa.net/detail/BIM-480681
Modern Language Association (MLA)
Prabhu, Pranav R.& Hudson, André O.. Identification and Partial Characterization of an L-Tyrosine Aminotransferase (TAT) from Arabidopsis thaliana. Biochemistry Research International No. 2010 (2010), pp.1-11.
https://search.emarefa.net/detail/BIM-480681
American Medical Association (AMA)
Prabhu, Pranav R.& Hudson, André O.. Identification and Partial Characterization of an L-Tyrosine Aminotransferase (TAT) from Arabidopsis thaliana. Biochemistry Research International. 2010. Vol. 2010, no. 2010, pp.1-11.
https://search.emarefa.net/detail/BIM-480681
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-480681