Chondrocyte Behavior on Micropatterns Fabricated Using Layer-by-Layer Lift-Off : Morphological Analysis
Joint Authors
McShane, Michael J.
Shaikh Mohammed, Javeed
Mills, David K.
Shaik, Jameel
Source
Journal of Medical Engineering
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-05-28
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Cell patterning has emerged as an elegant tool in developing cellular arrays, bioreactors, biosensors, and lab-on-chip devices and for use in engineering neotissue for repair or regeneration.
In this study, micropatterned surfaces were created using the layer-by-layer lift-off (LbL-LO) method for analyzing canine chondrocytes response to patterned substrates.
Five materials were chosen based on our previous studies.
These included: poly(dimethyldiallylammonium chloride) (PDDA), poly(ethyleneimine) (PEI), poly(styrene sulfonate) (PSS), collagen, and chondroitin sulfate (CS).
The substrates were patterned with these five different materials, in five and ten bilayers, resulting in the following multilayer nanofilm architectures: (PSS/PDDA)5, (PSS/PDDA)10; (CS/PEI)4/CS, (CS/PEI)9/CS; (PSS/PEI)5, (PSS/PEI)10; (PSS/Collagen)5, (PSS/Collagen)10; (PSS/PEI)4/PSS, (PSS/PEI)9/PSS.
Cell characterization studies were used to assess the viability, longevity, and cellular response to the configured patterned multilayer architectures.
The cumulative cell characterization data suggests that cell viability, longevity, and functionality were enhanced on micropatterned PEI, PSS, collagen, and CS multilayer nanofilms suggesting their possible use in biomedical applications.
American Psychological Association (APA)
Shaik, Jameel& Shaikh Mohammed, Javeed& McShane, Michael J.& Mills, David K.. 2013. Chondrocyte Behavior on Micropatterns Fabricated Using Layer-by-Layer Lift-Off : Morphological Analysis. Journal of Medical Engineering،Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-480755
Modern Language Association (MLA)
Shaik, Jameel…[et al.]. Chondrocyte Behavior on Micropatterns Fabricated Using Layer-by-Layer Lift-Off : Morphological Analysis. Journal of Medical Engineering No. 2013 (2013), pp.1-12.
https://search.emarefa.net/detail/BIM-480755
American Medical Association (AMA)
Shaik, Jameel& Shaikh Mohammed, Javeed& McShane, Michael J.& Mills, David K.. Chondrocyte Behavior on Micropatterns Fabricated Using Layer-by-Layer Lift-Off : Morphological Analysis. Journal of Medical Engineering. 2013. Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-480755
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-480755