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Increasing the Productivity of Glycopeptides Analysis by Using Higher-Energy Collision Dissociation-Accurate Mass-Product-Dependent Electron Transfer Dissociation
Joint Authors
Saba, Julian
Dutta, Sucharita
Hemenway, Eric
Viner, Rosa
Source
International Journal of Proteomics
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-05-30
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
Currently, glycans are attracting attention from the scientific community as potential biomarkers or as posttranslational modifications (PTMs) of therapeutic proteins.
However, structural characterization of glycoproteins and glycopeptides remains analytically challenging.
Here, we report on the implementation of a novel acquisition strategy termed higher-energy collision dissociation-accurate mass-product-dependent electron transfer dissociation (HCD-PD-ETD) on a hybrid linear ion trap-orbitrap mass spectrometer.
This acquisition strategy uses the complementary fragmentations of ETD and HCD for glycopeptides analysis in an intelligent fashion.
Furthermore, the approach minimizes user input for optimizing instrumental parameters and enables straightforward detection of glycopeptides.
ETD spectra are only acquired when glycan oxonium ions from MS/MS HCD are detected.
The advantage of this approach is that it streamlines data analysis and improves dynamic range and duty cycle.
Here, we present the benefits of HCD-PD-ETD relative to the traditional alternating HCD/ETD for a trainer set containing twelve-protein mixture with two glycoproteins: human serotransferrin, ovalbumin and contaminations of two other: bovine alpha 1 acid glycoprotein (bAGP) and bovine fetuin.
American Psychological Association (APA)
Saba, Julian& Dutta, Sucharita& Hemenway, Eric& Viner, Rosa. 2012. Increasing the Productivity of Glycopeptides Analysis by Using Higher-Energy Collision Dissociation-Accurate Mass-Product-Dependent Electron Transfer Dissociation. International Journal of Proteomics،Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-480763
Modern Language Association (MLA)
Saba, Julian…[et al.]. Increasing the Productivity of Glycopeptides Analysis by Using Higher-Energy Collision Dissociation-Accurate Mass-Product-Dependent Electron Transfer Dissociation. International Journal of Proteomics No. 2012 (2012), pp.1-7.
https://search.emarefa.net/detail/BIM-480763
American Medical Association (AMA)
Saba, Julian& Dutta, Sucharita& Hemenway, Eric& Viner, Rosa. Increasing the Productivity of Glycopeptides Analysis by Using Higher-Energy Collision Dissociation-Accurate Mass-Product-Dependent Electron Transfer Dissociation. International Journal of Proteomics. 2012. Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-480763
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-480763