Partial Characterization of a Vicilin-Like Glycoprotein from Seeds of Flowering Tobacco (Nicotiana sylvestris)
Joint Authors
Haynes, Paul A.
Bhavanandan, Veer P.
Joshi, Lokesh
Gerlach, Jared Q.
Source
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2009-06-14
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
A vicilin-like glycoprotein from the seeds of Nicotiana sylvestris, flowering tobacco, has been identified using nanoLC/ESI-MS/MS.
Sequences from a fragment of protein demonstrated homology with vicilins from other members of the Solanaceae family, notably potato (Solanum demissum).
Reducing and nonreducing SDS-PAGE analyses of the identified protein indicated that fragments resulting from in situ proteolytic processing are joined by intrachain disulphide bonds.
Staining with Con A lectin was specifically inhibited by mannose suggested the presence of N-linked glycosylation which was confirmed by carbohydrate compositional analysis of PVDF-bound protein subunits.
HPAEC-PAD analysis of the monosaccharides released from the glycoprotein by acid hydrolysis revealed glucosamine and mannose.
N-acetylglucosamine termination of attached oligosaccharides was further verified by inhibitable WGA lectin staining.
Immunostaining of PVDF-bound N.
sylvestris proteins with antibodies against G.
max total protein demonstrated cross-staining at masses corresponding to fragments from the proteolytically processed protein subunits.
American Psychological Association (APA)
Gerlach, Jared Q.& Bhavanandan, Veer P.& Haynes, Paul A.& Joshi, Lokesh. 2009. Partial Characterization of a Vicilin-Like Glycoprotein from Seeds of Flowering Tobacco (Nicotiana sylvestris). Journal of Botany،Vol. 2009, no. 2009, pp.1-12.
https://search.emarefa.net/detail/BIM-480764
Modern Language Association (MLA)
Gerlach, Jared Q.…[et al.]. Partial Characterization of a Vicilin-Like Glycoprotein from Seeds of Flowering Tobacco (Nicotiana sylvestris). Journal of Botany No. 2009 (2009), pp.1-12.
https://search.emarefa.net/detail/BIM-480764
American Medical Association (AMA)
Gerlach, Jared Q.& Bhavanandan, Veer P.& Haynes, Paul A.& Joshi, Lokesh. Partial Characterization of a Vicilin-Like Glycoprotein from Seeds of Flowering Tobacco (Nicotiana sylvestris). Journal of Botany. 2009. Vol. 2009, no. 2009, pp.1-12.
https://search.emarefa.net/detail/BIM-480764
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-480764