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Simple Detection of Large InDeLS by DHPLC : The ACE Gene as a Model
Joint Authors
Tufik, Sergio
Pedrazzoli, Mario
Koyama, Renata Guedes
De Mello, Marco Túlio
Castro, Rosa M. R. P. S.
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2008, Issue 2008 (31 Dec. 2008), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2008-04-20
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
Insertion-deletion polymorphism (InDeL) is the second most frequent type of genetic variation in the human genome.
For the detection of large InDeLs, researchers usually resort to either PCR gel analysis or RFLP, but these are time consuming and dependent on human interpretation.
Therefore, a more efficient method for genotyping this kind of genetic variation is needed.
In this report, we describe a method that can detect large InDeLs by DHPLC (denaturating high-performance liquid chromatography) using the angiotensin-converting enzyme (ACE) gene I/D polymorphism as a model.
The InDeL targeted in this study is characterized by a 288 bp Alu element insertion (I).
We used DHPLC at nondenaturating conditions to analyze the PCR product with a flow through the chromatographic column under two different gradients based on the differences between D and I sequences.
The analysis described is quick and easy, making this technique a suitable and efficient means for DHPLC users to screen InDeLs in genetic epidemiological studies.
American Psychological Association (APA)
Koyama, Renata Guedes& Castro, Rosa M. R. P. S.& De Mello, Marco Túlio& Tufik, Sergio& Pedrazzoli, Mario. 2008. Simple Detection of Large InDeLS by DHPLC : The ACE Gene as a Model. Journal of Biomedicine and Biotechnology،Vol. 2008, no. 2008, pp.1-5.
https://search.emarefa.net/detail/BIM-480933
Modern Language Association (MLA)
Koyama, Renata Guedes…[et al.]. Simple Detection of Large InDeLS by DHPLC : The ACE Gene as a Model. Journal of Biomedicine and Biotechnology No. 2008 (2008), pp.1-5.
https://search.emarefa.net/detail/BIM-480933
American Medical Association (AMA)
Koyama, Renata Guedes& Castro, Rosa M. R. P. S.& De Mello, Marco Túlio& Tufik, Sergio& Pedrazzoli, Mario. Simple Detection of Large InDeLS by DHPLC : The ACE Gene as a Model. Journal of Biomedicine and Biotechnology. 2008. Vol. 2008, no. 2008, pp.1-5.
https://search.emarefa.net/detail/BIM-480933
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-480933