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Biochemical Aspects of a Serine Protease from Caesalpinia echinata Lam. (Brazilwood) Seeds : A Potential Tool to Access the Mobilization of Seed Storage Proteins
Joint Authors
Tanaka, Aparecida Sadae
Gozzo, Andrezza Justino
Figueiredo-Ribeiro, Rita de Cássia
Cruz-Silva, Ilana
Araújo, Mariana da Silva
Abreu Nunes, Viviane
Torquato, Ricardo José
Praxedes-Garcia, Priscila
González, Yamile González
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-05-02
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Medicine
Information Technology and Computer Science
Abstract EN
Several proteins have been isolated from seeds of leguminous, but this is the first report that a protease was obtained from seeds of Caesalpinia echinata Lam., a tree belonging to the Fabaceae family.
This enzyme was purified to homogeneity by hydrophobic interaction and anion exchange chromatographies and gel filtration.
This 61-kDa serine protease (CeSP) hydrolyses H-D-prolyl-L-phenylalanyl-L-arginine-p-nitroanilide (Km 55.7 μM) in an optimum pH of 7.1, and this activity is effectively retained until 50∘C.
CeSP remained stable in the presence of kosmotropic anions (PO4 3−, SO4 2−, and CH3COO−) or chaotropic cations (K+ and Na+).
It is strongly inhibited by TLCK, a serine protease inhibitor, but not by E-64, EDTA or pepstatin A.
The characteristics of the purified enzyme allowed us to classify it as a serine protease.
The role of CeSP in the seeds cannot be assigned yet but is possible to infer that it is involved in the mobilization of seed storage proteins.
American Psychological Association (APA)
Praxedes-Garcia, Priscila& Cruz-Silva, Ilana& Gozzo, Andrezza Justino& Abreu Nunes, Viviane& Torquato, Ricardo José& Tanaka, Aparecida Sadae…[et al.]. 2012. Biochemical Aspects of a Serine Protease from Caesalpinia echinata Lam. (Brazilwood) Seeds : A Potential Tool to Access the Mobilization of Seed Storage Proteins. The Scientific World Journal،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-480982
Modern Language Association (MLA)
Praxedes-Garcia, Priscila…[et al.]. Biochemical Aspects of a Serine Protease from Caesalpinia echinata Lam. (Brazilwood) Seeds : A Potential Tool to Access the Mobilization of Seed Storage Proteins. The Scientific World Journal No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-480982
American Medical Association (AMA)
Praxedes-Garcia, Priscila& Cruz-Silva, Ilana& Gozzo, Andrezza Justino& Abreu Nunes, Viviane& Torquato, Ricardo José& Tanaka, Aparecida Sadae…[et al.]. Biochemical Aspects of a Serine Protease from Caesalpinia echinata Lam. (Brazilwood) Seeds : A Potential Tool to Access the Mobilization of Seed Storage Proteins. The Scientific World Journal. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-480982
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-480982