Priming DNA Replication from Triple Helix Oligonucleotides : Possible Threestranded DNA in DNA Polymerases
Author
Source
Molecular Biology International
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-09-14
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
Triplex associate with a duplex DNA presenting the same polypurine or polypyrimidine-rich sequence in an antiparallel orientation.
So far, triplex forming oligonucleotides (TFOs) are known to inhibit transcription, replication, and to induce mutations.
A new property of TFO is reviewed here upon analysis of DNA breakpoint yielding DNA rearrangements; the synthesized sequence of the first direct repeat displays a skewed polypurine- rich sequence.
This synthesized sequence can bind the second homologous duplex sequence through the formation of a triple helix, which is able to prime further DNA replication.
In these case, the d(G)-rich Triple Helix Primers (THP) bind the homologous strand in a parallel manner, possibly via a RecA-like mechanism.
This novel property is shared by all tested DNA polymerases: phage, retrovirus, bacteria, and human.
These features may account for illegitimate initiation of replication upon single-strand breakage and annealing to a homologous sequence where priming may occur.
Our experiments suggest that DNA polymerases can bind three instead of two polynucleotide strands in their catalytic centre.
American Psychological Association (APA)
Lestienne, Patrick P.. 2011. Priming DNA Replication from Triple Helix Oligonucleotides : Possible Threestranded DNA in DNA Polymerases. Molecular Biology International،Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-480996
Modern Language Association (MLA)
Lestienne, Patrick P.. Priming DNA Replication from Triple Helix Oligonucleotides : Possible Threestranded DNA in DNA Polymerases. Molecular Biology International No. 2011 (2011), pp.1-9.
https://search.emarefa.net/detail/BIM-480996
American Medical Association (AMA)
Lestienne, Patrick P.. Priming DNA Replication from Triple Helix Oligonucleotides : Possible Threestranded DNA in DNA Polymerases. Molecular Biology International. 2011. Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-480996
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-480996