Immunogenicity and Protective Efficacy of a Novel Recombinant BCG Strain Overexpressing Antigens Ag85A and Ag85B
Joint Authors
Shi, Chunwei
Fu, Ruiling
Fan, Xionglin
Wang, Chun
Tan, Kun
Chen, Zhenhua
Chen, Lingxia
Teng, Xindong
Lu, Jia
Source
Clinical and Developmental Immunology
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-04-18
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Recombinant Bacillus Calmette-Guérin (rBCG) strain is the promising vaccine candidate for tuberculosis (TB) prevention, which aims at providing more enduring and enhanced protection than the parental BCG vaccine.
In this study, three rBCG strains overexpressing immunodominant antigens Ag85B (rBCG::85B), Ag85A (rBCG::85A), or both (rBCG::AB) of Mycobacterium tuberculosis were constructed, respectively.
rBCG strains showed higher level of overexpression of Ag85A and/or Ag85B proteins than BCG containing empty vector pMV261(rBCG::261), which had low levels of endogenous expression of both proteins as expected.
rBCG::AB strain could provide the strongest short-term and long-term protection in the lung against intravenous infection with virulent M.
tuberculosis than rBCG::261 control and other two rBCG strains overexpressing single antigen.
The stronger and longer-lasting protection provided by rBCG::AB than rBCG::261 was correlated with systemic in vitro antigen-specific IFN-γ responses.
Therefore, our results indicate that rBCG::AB could be a very promising TB vaccine candidate and should be further evaluated for the preclinical test.
American Psychological Association (APA)
Wang, Chun& Fu, Ruiling& Chen, Zhenhua& Tan, Kun& Chen, Lingxia& Teng, Xindong…[et al.]. 2012. Immunogenicity and Protective Efficacy of a Novel Recombinant BCG Strain Overexpressing Antigens Ag85A and Ag85B. Clinical and Developmental Immunology،Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-481081
Modern Language Association (MLA)
Wang, Chun…[et al.]. Immunogenicity and Protective Efficacy of a Novel Recombinant BCG Strain Overexpressing Antigens Ag85A and Ag85B. Clinical and Developmental Immunology No. 2012 (2012), pp.1-9.
https://search.emarefa.net/detail/BIM-481081
American Medical Association (AMA)
Wang, Chun& Fu, Ruiling& Chen, Zhenhua& Tan, Kun& Chen, Lingxia& Teng, Xindong…[et al.]. Immunogenicity and Protective Efficacy of a Novel Recombinant BCG Strain Overexpressing Antigens Ag85A and Ag85B. Clinical and Developmental Immunology. 2012. Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-481081
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-481081