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The Assessment of Viability of M. Tuberculosis after Exposure to CPC Using Different Methods
Joint Authors
Sekar, Gomathi
Lakshmi, R.
Selvakumar, N.
Source
International Journal of Bacteriology
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-01-28
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
Settings.
National Institute for Research in Tuberculosis, Chennai.
Objective.
To assess the proportion of metabolically active cells of Mycobacterium tuberculosis after exposed to CPC using FDA-EB vital staining and viable counts on LJ medium.
Mycolic acid content in M.
tuberculosis after exposure to CPC was estimated using HPLC.
Methods.
Clinical isolates of M.
tuberculosis and standard reference strain M.
tuberculosis H37Rv were used for FDA-EB, viable count, and HPLC.
Results.
FDA/EB consistently stained 70–90% of log phase cells as green and the remaining cells as red-orange.
After CPC treatment, 65–70% of the cells stained red-orange.
The viability counts were comparable to 0-day controls.
Synthesis of mycolic acids in mycobacteria was reduced when exposed to CPC using HPLC due to the decreased metabolic activity of the organisms.
Conclusion.
The cells are metabolically inactive during storage with CPC but these cells grew well on LJ medium after removal of CPC.
The viability of M.
tuberculosis was maintained in CPC with minimal reduction.
Mycolic acid content was reduced if the cells of M.
tuberculosis were treated with CPC for 7 days.
All the above findings provide yet another evidence for the damage of cell wall of M.
tuberculosis.
American Psychological Association (APA)
Sekar, Gomathi& Lakshmi, R.& Selvakumar, N.. 2014. The Assessment of Viability of M. Tuberculosis after Exposure to CPC Using Different Methods. International Journal of Bacteriology،Vol. 2014, no. 2014, pp.1-5.
https://search.emarefa.net/detail/BIM-481106
Modern Language Association (MLA)
Sekar, Gomathi…[et al.]. The Assessment of Viability of M. Tuberculosis after Exposure to CPC Using Different Methods. International Journal of Bacteriology No. 2014 (2014), pp.1-5.
https://search.emarefa.net/detail/BIM-481106
American Medical Association (AMA)
Sekar, Gomathi& Lakshmi, R.& Selvakumar, N.. The Assessment of Viability of M. Tuberculosis after Exposure to CPC Using Different Methods. International Journal of Bacteriology. 2014. Vol. 2014, no. 2014, pp.1-5.
https://search.emarefa.net/detail/BIM-481106
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-481106