Micellar LC Separation of Sesquiterpenic Acids and Their Determination in Valeriana officinalis L. Root and Extracts
Author
Source
Chromatography Research International
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-04-10
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
A simple micellar liquid chromatography (MLC) method was developed and validated according to ICH Guidelines for the determination of sesquiterpenic acids (valerenic, hydroxyvalerenic, and acetoxyvalerenic acids) in root and rhizome extract from Valeriana officinalis L.
and valerian dry hydroalcoholic extract.
Samples were analyzed on Nucleosil C18 column (150 mm × 4.6 mm, 5 μm) using an isocratic mobile phase which consisted of Brij 35 (5% (w/v) aqueous solution; pH 2.3±0.1 by phosphoric acid) and 1-butanol (6% (v/v)); UV detection was at 220 nm.
Micellar mobile phase using allows to fully separate valerenic acids within 25 minutes.
Linearity for hydroxyvalerenic, acetoxyvalerenic, and valerenic acids was 1.9–27.9, 4.2–63.0, and 6.1–91⋅3 μg.mL-1, and limit of detection was 0.14, 0.037, and 0.09 μg⋅mL-1, respectively.
Intraday and interday precisions were not less than 2% for all investigated compounds.
The proposed method was found to be reproducible and convenient for quantitative analysis of sesquiterpenic acids in valerian root and related preparations.
American Psychological Association (APA)
Kulikov, Artem U.. 2012. Micellar LC Separation of Sesquiterpenic Acids and Their Determination in Valeriana officinalis L. Root and Extracts. Chromatography Research International،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-481114
Modern Language Association (MLA)
Kulikov, Artem U.. Micellar LC Separation of Sesquiterpenic Acids and Their Determination in Valeriana officinalis L. Root and Extracts. Chromatography Research International No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-481114
American Medical Association (AMA)
Kulikov, Artem U.. Micellar LC Separation of Sesquiterpenic Acids and Their Determination in Valeriana officinalis L. Root and Extracts. Chromatography Research International. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-481114
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-481114