Whole Cell Biosensor Using Anabaena torulosa with Optical Transduction for Environmental Toxicity Evaluation
Joint Authors
Wong, Ling Shing
Heng, Lee Yook
Surif, Salmijah
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-07-21
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
A whole cell-based biosensor using Anabaena torulosa for the detection of heavy metals (Cu, Pb, and Cd), 2,4-dichlorophenoxyacetate (2,4-D), and chlorpyrifos was constructed.
The cyanobacteria were entrapped on a cellulose membrane through filtration.
Then, the membrane was dried and fixed into a cylindrical well, which was designed to be attached to an optical probe.
The probe was connected to fluorescence spectrometer with optical fibre.
The presence of the toxicants was indicated by the change of fluorescence emission, before and after the exposure.
The linear detection ranges for Cu, Pb, and Cd were 2.5–10.0 µg/L, 0.5–5.0 µg/L, and 0.5–10.0 µg/L, respectively, while 2,4-D and chlorpyrifos shared similar linear ranges of 0.05–0.75 µg/L.
The biosensor showed good sensitivity with the lowest limits of detection (LLD) for Cu, Pb, Cd, 2,4-D and chlorpyrifos determined at 1.195 µg/L, 0.100 µg/L, 0.027 µg/L, 0.025 µg/L, and 0.025 µg/L, respectively.
The overall reproducibility of the biosensor (n=3) was <±6.35%.
The biosensor had been tested with different combinations of toxicants, with the results showing predominantly antagonistic responses.
The results confirmed that the biosensor constructed in this report is suitable to be used in quantitative and qualitative detections of heavy metals and pesticides.
American Psychological Association (APA)
Wong, Ling Shing& Heng, Lee Yook& Surif, Salmijah. 2013. Whole Cell Biosensor Using Anabaena torulosa with Optical Transduction for Environmental Toxicity Evaluation. Journal of Sensors،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-481292
Modern Language Association (MLA)
Wong, Ling Shing…[et al.]. Whole Cell Biosensor Using Anabaena torulosa with Optical Transduction for Environmental Toxicity Evaluation. Journal of Sensors No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-481292
American Medical Association (AMA)
Wong, Ling Shing& Heng, Lee Yook& Surif, Salmijah. Whole Cell Biosensor Using Anabaena torulosa with Optical Transduction for Environmental Toxicity Evaluation. Journal of Sensors. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-481292
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-481292